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验证数据展示

  • WB analysis using 83465-2-RR

    WB analysis using 83465-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83465-2-RR (GRIN2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of rat brain using 83465-2-RR

    IF Staining of rat brain using 83465-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NMDAR2A/GRIN2A antibody (83465-2-RR, Clone: 240445D4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat brain using 83465-2-RR

    IF Staining of rat brain using 83465-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NMDAR2A/GRIN2A antibody (83465-2-RR, Clone: 240445D4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of U-251 using 83465-2-RR

    FC experiment of U-251 using 83465-2-RR

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug Grin2a Recombinant Antibody (83465-2-RR, Clone:240445D4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83465-2-RR

    Affinity and Kinetic Characterization of 83465-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83465-2-RR against Human NMDAR2A/GRIN2A were performed. The affinity constant is 11.0 nM.

NMDAR2A/GRIN2A Recombinant antibody

NMDAR2A/GRIN2A Recombinant Antibody for WB, IF-P, FC (Intra), ELISA
Cat No. 83465-2-RR
CloneNo. 240445D4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, FC (Intra), ELISA

Synonyms

GRIN2A, NMDAR2A, hNR2A, Glutamate receptor ionotropic, NMDA 2A, Glutamate [NMDA] receptor subunit epsilon-1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF-P detected inrat brain tissue
Positive FC (Intra) detected inU-251 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83465-2-RR targets NMDAR2A/GRIN2A in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF-P, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NMDAR2A/GRIN2A fusion protein Ag29101 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name glutamate receptor, ionotropic, N-methyl D-aspartate 2A
Synonyms GRIN2A, NMDAR2A, hNR2A, Glutamate receptor ionotropic, NMDA 2A, Glutamate [NMDA] receptor subunit epsilon-1
Calculated Molecular Weight 165 kDa
Observed Molecular Weight160-180 kDa
GenBank Accession NumberNM_000833
Gene Symbol GRIN2A
Gene ID (NCBI) 2903
RRIDAB_3671095
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ12879
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GRIN2A (glutamate ionotropic receptor NMDA type subunit 2A), also known as NMDAR2A. And its molecular weight is 165 kDa. GRIN2A is located in cell projection, dendritic spine, cell membrane, synapse, postsynaptic cell membrae, cytolamic vesicle membrane, which is expressed in many tissues, highest expression in brain and heart. This gene encodes a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. These receptors are permeable to calcium ions, and activation results in a calcium influx into post-synaptic cells, which results in the activation of several signaling cascades. Disruption of this gene is associated with focal epilepsy and speech disorder with or without cognitive disability. Alternative splicing results in multiple transcript variants.

实验方案

Product Specific Protocols
WB protocol for NMDAR2A/GRIN2A antibody 83465-2-RRDownload protocol
IF protocol for NMDAR2A/GRIN2A antibody 83465-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols