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NMDAR2A/GRIN2A Recombinant antibody, PBS Only (Detector)

NMDAR2A/GRIN2A Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240445D4

Cat no : 83465-2-PBS

Print datasheet

Synonyms

GRIN2A, NMDAR2A, hNR2A, Glutamate receptor ionotropic, NMDA 2A, Glutamate [NMDA] receptor subunit epsilon-1

验证数据展示

  • WB analysis using 83465-2-RR (same clone as 83465-2-PBS)

    WB analysis using 83465-2-RR (same clone as 83465-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83465-2-RR (GRIN2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83465-2-PBS in a different storage buffer formulation.

  • FC experiment of U-251 using 83465-2-RR (same clone as 83465-2-PBS)

    FC experiment of U-251 using 83465-2-RR (same clone as 83465-2-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug Grin2a Recombinant Antibody (83465-2-RR, Clone:240445D4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83465-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00461-2

    Cytometric bead array standard curve of MP00461-2

    Cytometric bead array standard curve of MP00461-2, GRIN2A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83465-4-PBS. Detection antibody: 83465-2-PBS. Standard: Ag29101. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83465-2-PBS

    Affinity and Kinetic Characterization of 83465-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 83465-2-PBS against Human NMDAR2A/GRIN2A was performed. The affinity constant is 7.26 nM.

  • Affinity and Kinetic Characterization of 83465-2-RR

    Affinity and Kinetic Characterization of 83465-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83465-2-RR against Human NMDAR2A/GRIN2A were performed. The affinity constant is 11.0 nM.

产品信息

83465-2-PBS targets NMDAR2A/GRIN2A as part of a matched antibody pair:

MP00461-2: 83465-4-PBS capture and 83465-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NMDAR2A/GRIN2A fusion protein Ag29101 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name glutamate receptor, ionotropic, N-methyl D-aspartate 2A
Synonyms GRIN2A, NMDAR2A, hNR2A, Glutamate receptor ionotropic, NMDA 2A, Glutamate [NMDA] receptor subunit epsilon-1
Calculated Molecular Weight 165 kDa
Observed Molecular Weight160-180 kDa
GenBank Accession NumberNM_000833
Gene Symbol GRIN2A
Gene ID (NCBI) 2903
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ12879
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GRIN2A (glutamate ionotropic receptor NMDA type subunit 2A), also known as NMDAR2A. And its molecular weight is 165 kDa. GRIN2A is located in cell projection, dendritic spine, cell membrane, synapse, postsynaptic cell membrae, cytolamic vesicle membrane, which is expressed in many tissues, highest expression in brain and heart. This gene encodes a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. These receptors are permeable to calcium ions, and activation results in a calcium influx into post-synaptic cells, which results in the activation of several signaling cascades. Disruption of this gene is associated with focal epilepsy and speech disorder with or without cognitive disability. Alternative splicing results in multiple transcript variants.