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验证数据展示

  • WB analysis using 85074-2-RR

    WB analysis using 85074-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85074-2-RR (NME2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HEK-293T using 85074-2-RR

    FC experiment of HEK-293T using 85074-2-RR

    1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug NME2 Recombinant antibody (85074-2-RR, Clone:242698C7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

NME2 Recombinant antibody

NME2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 85074-2-RR
CloneNo. 242698C7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

NDKB, Histidine protein kinase NDKB, EC:2.7.4.6, EC:2.7.13.3, C-myc purine-binding transcription factor PUF

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse kidney tissue, mouse liver tissue
Positive FC (Intra) detected inHEK-293T cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85074-2-RR targets NME2 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen NME2 fusion protein Ag13913 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name non-metastatic cells 2, protein (NM23B) expressed in
Synonyms NDKB, Histidine protein kinase NDKB, EC:2.7.4.6, EC:2.7.13.3, C-myc purine-binding transcription factor PUF
Calculated Molecular Weight 152 aa, 17 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC002476
Gene Symbol NME2
Gene ID (NCBI) 4831
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP22392
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NME2(non-metastatic cells 2, protein) is also named as NDKB(Nucleoside diphosphate kinase B), NM23B and belongs to the NDK family. It is involved in the phosphorylation of nucleoside diphosphates and interacts with membrane receptor and constituting a novel molecular regulatory mechanism of GPCR endocytosis. This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for NME2 antibody 85074-2-RRDownload protocol
FC protocol for NME2 antibody 85074-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols