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NMI Recombinant antibody, PBS Only

NMI Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241135H2

Cat no : 83986-5-PBS

Print datasheet

Synonyms

N-myc-interactor, N-myc and STAT interactor, N myc interactor, N myc and STAT interactor, N myc (and STAT) interactor

验证数据展示

  • WB analysis using 83986-5-RR (same clone as 83986-5-PBS)

    WB analysis using 83986-5-RR (same clone as 83986-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83986-5-RR (NMI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83986-5-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83986-5-RR (same clone as 83986-5-PBS)

    FC experiment of HepG2 using 83986-5-RR (same clone as 83986-5-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NMI Recombinant antibody (83986-5-RR, Clone:241135H2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83986-5-PBS in a different storage buffer formulation.

产品信息

83986-5-PBS targets NMI in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen NMI fusion protein Ag34759 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name N-myc (and STAT) interactor
Synonyms N-myc-interactor, N-myc and STAT interactor, N myc interactor, N myc and STAT interactor, N myc (and STAT) interactor
Calculated Molecular Weight 35 kDa
Observed Molecular Weight37 kDa
GenBank Accession NumberBC001268
Gene Symbol NMI
Gene ID (NCBI) 9111
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ13287
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

N-Myc and STAT Interactor protein (NMI) is an interferon inducible protein that interacts with NMYC and CMYC (members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. It is widely involved in the process of tumor growth, progression, and metastasis.