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NMI Recombinant antibody

NMI Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241135H2

Cat no : 83986-5-RR

Print datasheet

Synonyms

N-myc-interactor, N-myc and STAT interactor, N myc interactor, N myc and STAT interactor, N myc (and STAT) interactor

验证数据展示

  • WB analysis using 83986-5-RR

    WB analysis using 83986-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83986-5-RR (NMI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 83986-5-RR

    FC experiment of HepG2 using 83986-5-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NMI Recombinant antibody (83986-5-RR, Clone:241135H2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, PC-3 cells, HepG2 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83986-5-RR targets NMI in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen NMI fusion protein Ag34759 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name N-myc (and STAT) interactor
Synonyms N-myc-interactor, N-myc and STAT interactor, N myc interactor, N myc and STAT interactor, N myc (and STAT) interactor
Calculated Molecular Weight 35 kDa
Observed Molecular Weight37 kDa
GenBank Accession NumberBC001268
Gene Symbol NMI
Gene ID (NCBI) 9111
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ13287
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

N-Myc and STAT Interactor protein (NMI) is an interferon inducible protein that interacts with NMYC and CMYC (members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. It is widely involved in the process of tumor growth, progression, and metastasis.

实验方案

Product Specific Protocols
WB protocol for NMI antibody 83986-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols