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NOP58 Recombinant antibody

NOP58 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

230058C7

Cat No : 82879-1-RR

Print datasheet

Synonyms

Nucleolar protein 5, NOP5/NOP58, NOP5, NOL5, HSPC120

验证数据展示

  • WB analysis using 82879-1-RR

    WB analysis using 82879-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82879-1-RR (NOP58 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 82879-1-RR

    IP experiment of HeLa using 82879-1-RR

    IP result of anti-NOP58 (IP:82879-1-RR, 4ug; Detection:82879-1-RR 1:2000) with HeLa cells lysate 1600 ug.

  • IHC staining of human prostate cancer using 82879-1-RR

    IHC staining of human prostate cancer using 82879-1-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82879-1-RR (NOP58 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NOP58 antibody included
  • IF Staining of U2OS using 82879-1-RR

    IF Staining of U2OS using 82879-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NOP58 antibody (82879-1-RR) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

  • FC experiment of HeLa using 82879-1-RR

    FC experiment of HeLa using 82879-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug NOP58 Recombinant antibody (82879-1-RR, Clone:230058C7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 82879-1-RR

    Affinity and Kinetic Characterization of 82879-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82879-1-RR against Human NOP58 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, MCF-7 cells, HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NOP58 Ready-To-Use IHC Kit. NOP58 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82879-1-RR targets NOP58 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Tested Reactivity Human
Immunogen NOP58 fusion protein Ag5740 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name NOP58 ribonucleoprotein homolog (yeast)
Synonyms Nucleolar protein 5, NOP5/NOP58, NOP5, NOL5, HSPC120
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC032592
Gene Symbol NOP58
Gene ID (NCBI) 51602
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y2X3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The box C/D snoRNPs are named for the short, conserved box C (UGAUGA) and box D (CUGA) sequences present in their RNA moiety. The box C/D sequences are also required for multiple aspects. of snoRNA biogenesis, including RNA stability, intronic processing, nucleolar targeting, nuclear retention, and 59 trimethylguanosine cap formation (PMID:9649444,8878486). Nop58 is a component common to the box C/D small nucleolar ribonucleoprotein (PMID:10606270).

实验方案

Product Specific Protocols
WB protocol for NOP58 antibody 82879-1-RRDownload protocol
IHC protocol for NOP58 antibody 82879-1-RRDownload protocol
IF protocol for NOP58 antibody 82879-1-RRDownload protocol
IP protocol for NOP58 antibody 82879-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols