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CoraLite® Plus 488-conjugated NOP58 Recombinant antibody

NOP58 Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230058C7

Cat No : CL488-82879

Print datasheet

Synonyms

Nucleolar protein 5, NOP5/NOP58, NOP5, NOL5, HSPC120

验证数据展示

  • IF Staining of U2OS using CL488-82879

    IF Staining of U2OS using CL488-82879

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 NOP58 antibody (CL488-82879, Clone: 230058C7 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-82879

    FC experiment of HeLa using CL488-82879

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated NOP58 Recombinant antibody (CL488-82879, Clone:230058C7)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NOP58 Ready-To-Use IHC Kit. NOP58 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 82879-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82879 targets NOP58 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen NOP58 fusion protein Ag5740 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name NOP58 ribonucleoprotein homolog (yeast)
Synonyms Nucleolar protein 5, NOP5/NOP58, NOP5, NOL5, HSPC120
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC032592
Gene Symbol NOP58
Gene ID (NCBI) 51602
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y2X3
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The box C/D snoRNPs are named for the short, conserved box C (UGAUGA) and box D (CUGA) sequences present in their RNA moiety. The box C/D sequences are also required for multiple aspects. of snoRNA biogenesis, including RNA stability, intronic processing, nucleolar targeting, nuclear retention, and 59 trimethylguanosine cap formation (PMID:9649444,8878486). Nop58 is a component common to the box C/D small nucleolar ribonucleoprotein (PMID:10606270).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NOP58 antibody CL488-82879Download protocol
FC protocol for CL Plus 488 NOP58 antibody CL488-82879Download protocol
Standard Protocols
Click here to view our Standard Protocols