- Featured Product
- KD/KO Validated
CoraLite® Plus 488-conjugated NOTCH3 Polyclonal antibody
NOTCH3 Polyclonal Antibody for
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
For other applications, we recommend the unconjugated version of this antibody, 55114-1-AP
产品信息
CL488-55114 targets NOTCH3 in applications and shows reactivity with human, mouse samples.
Tested Applications | Application Description |
Tested Reactivity | human, mouse |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | Notch homolog 3 (Drosophila) |
Synonyms | CADASIL, CASIL, Notch 3, Notch homolog 3 (Drosophila), NOTCH3 |
Calculated Molecular Weight | 244 kDa |
Observed Molecular Weight | 250-260 kDa, 100 kDa |
GenBank Accession Number | NM_000435 |
Gene Symbol | NOTCH3 |
Gene ID (NCBI) | 4854 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9UM47 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NOTCH3 belongs to the NOTCH family. It functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. NOTCH3 affects the implementation of differentiation, proliferation and apoptotic programs. Defects in NOTCH3 are the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). The antibody is specific to NOTCH3. Notch3 is a single-pass type I membrane protein exposed at the cell surface as a heterodimer of 2321 amino acids. It is synthesized as a precursor with a molecular weight of ~280 kDa (Notch3 full-length), which is cleaved (S1 cleavage) into a 210 kDa extracellular fragment and a 97 kDa intracellular fragment (PMID: 21702048).
实验方案
Product Specific Protocols | |
---|---|
FC protocol for CL Plus 488 NOTCH3 antibody CL488-55114 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |