• Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated NOTCH3 Polyclonal antibody

NOTCH3 Polyclonal Antibody for

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-55114

Print datasheet

Synonyms

CADASIL, CASIL, Notch 3, Notch homolog 3 (Drosophila), NOTCH3



For other applications, we recommend the unconjugated version of this antibody, 55114-1-AP

产品信息

CL488-55114 targets NOTCH3 in applications and shows reactivity with human, mouse samples.

Tested Applications Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Notch homolog 3 (Drosophila)
Synonyms CADASIL, CASIL, Notch 3, Notch homolog 3 (Drosophila), NOTCH3
Calculated Molecular Weight 244 kDa
Observed Molecular Weight 250-260 kDa, 100 kDa
GenBank Accession NumberNM_000435
Gene Symbol NOTCH3
Gene ID (NCBI) 4854
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UM47
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NOTCH3 belongs to the NOTCH family. It functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. NOTCH3 affects the implementation of differentiation, proliferation and apoptotic programs. Defects in NOTCH3 are the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). The antibody is specific to NOTCH3. Notch3 is a single-pass type I membrane protein exposed at the cell surface as a heterodimer of 2321 amino acids. It is synthesized as a precursor with a molecular weight of ~280 kDa (Notch3 full-length), which is cleaved (S1 cleavage) into a 210 kDa extracellular fragment and a 97 kDa intracellular fragment (PMID: 21702048).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 NOTCH3 antibody CL488-55114Download protocol
Standard Protocols
Click here to view our Standard Protocols