NOX1 Recombinant antibody

NOX1 Recombinant Antibody for WB, ELISA
Cat No. 84700-1-RR

产品说明书

CloneNo. 241935E7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

MOX1, MOX 1, Mitogenic oxidase 1, GP91 2, EC:1.6.3.-

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, COLO 320 cells, Caco-2 cells, mouse kidney tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84700-1-RR targets NOX1 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 NOX1 fusion protein Ag12187 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NADPH oxidase 1
别名 MOX1, MOX 1, Mitogenic oxidase 1, GP91 2, EC:1.6.3.-
计算分子量 564 aa, 65 kDa
观测分子量 59 kDa
GenBank蛋白编号BC075014
基因名称 NOX1
Gene ID (NCBI) 27035
RRIDAB_3672116
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9Y5S8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NNOX1(NADPH oxidase 1) is also named as MOX1, NOH1 and belongs to the reactive oxygen species (ROS)-generating NADPH oxidase family. It plays a role in host defense, cell growth and differentiation, cell migration, and malignant transformation(PMID:20351171). It also can mediate oncogenic Ras-induced upregulation of VEGF and angiogenesis by activating Sp1 through Ras-ERK-dependent phosphorylation of Sp1(PMID:18454179). It has 3 isoforms produced by alternative splicing with the molecular weight of 65 kDa, 22 kDa and 59 kDa. The full length protein has two glycosylation sites. It always can be detected two bands of 63 kDa and 55 kDa in cells by western blot or another lower band of 45 kDa which is not likely due to alternative translation or splice variation, but as adjustment for the signal peptide and tag removal(PMID:17560373) or unspecifc(PMID:17460729).

实验方案

Product Specific Protocols
WB protocol for NOX1 antibody 84700-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
Loading...