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验证数据展示

  • WB analysis using 82872-1-RR

    WB analysis using 82872-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82872-1-RR (CYBB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse spleen using 82872-1-RR

    IF Staining of mouse spleen using 82872-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using NOX2 antibody (82872-1-RR, Clone: 1B17 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of RAW 264.7 using 82872-1-RR

    FC experiment of RAW 264.7 using 82872-1-RR

    1x10^6 RAW 264.7 cells were intracellularly stained with 0.25 ug NOX2 Recombinant antibody (82872-1-RR, Clone:1B17) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of mouse spleen using 82872-1-RR

    IF Staining of mouse spleen using 82872-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using NOX2 antibody (82872-1-RR, Clone: 1B17 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NOX2 Recombinant antibody

NOX2 Recombinant Antibody for WB, IF-P, FC (Intra), ELISA
Cat No. 82872-1-RR

Print datasheet

CloneNo. 1B17

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, FC (Intra), ELISA

Synonyms

CYBB, EC:1.-.-.-, Cytochrome b-245 heavy chain, CGD91-phox, CGD

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse spleen tissue, rat spleen tissue
Positive IF-P detected inmouse spleen tissue
Positive FC (Intra) detected inRAW 264.7 cells
Planning an IHC experiment? We recommend our IHCeasy CYBB/NOX2 Ready-To-Use IHC Kit. CYBB/NOX2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)-PIF-P : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82872-1-RR targets NOX2 in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF-P, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NOX2 fusion protein Ag4402 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cytochrome b-245, beta polypeptide
Synonyms CYBB, EC:1.-.-.-, Cytochrome b-245 heavy chain, CGD91-phox, CGD
Calculated Molecular Weight 577 aa, 65 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC032720
Gene Symbol NOX2
Gene ID (NCBI) 1536
RRIDAB_3670592
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04839
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NOX2, also named as CYBB, CGD, 91-phox, gp91-1, gp91-phox, p22 phagocyte B-cytochrome, cytochrome b-245 and beta polypeptide, is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. This full length protein has three glycosylation sites. CYBB is found in human cardiomyocytes as multiple bands:the signal between 55 and 65 kDa is probably the unglycosylated protein, because the predicted molecular weight of unglycosylated CYBB protein is approximately 58 kDa(PMID: 17587483) to 65 kDa in phagocytes and the bands around 80 kDa probably represent glycosylated CYBB, as has also been shown in human umbilical vein endothelial cells(PMID:12610097). In other reports, it also can be detected a band of 91 kDa(PMID:19965781).

实验方案

Product Specific Protocols
WB protocol for NOX2 antibody 82872-1-RRDownload protocol
IF protocol for NOX2 antibody 82872-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols