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验证数据展示

  • WB analysis using 84230-5-RR

    WB analysis using 84230-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84230-5-RR (NPEPPS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 84230-5-RR

    IF Staining of A549 using 84230-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using NPEPPS antibody (84230-5-RR, Clone: 241502G1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of mouse liver using 84230-5-RR

    IHC staining of mouse liver using 84230-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84230-5-RR (NPEPPS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84230-5-RR

    Affinity and Kinetic Characterization of 84230-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84230-5-RR against Human NPEPPS were performed. The affinity constant is below 1 pM.

NPEPPS Recombinant antibody

NPEPPS Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84230-5-RR

产品说明书

CloneNo. 241502G1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

PSA, MP100, AAP S, 241502G1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HEK-29T cells, MDCK cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84230-5-RR targets NPEPPS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NPEPPS fusion protein Ag36035 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 aminopeptidase puromycin sensitive
别名 PSA, MP100, AAP S, 241502G1
观测分子量103 kda
GenBank蛋白编号BC065294
基因名称 NPEPPS
Gene ID (NCBI) 9520
RRIDAB_3671784
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP55786
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NPEPPS, also known as PSA, is a member of the oxytocinase subfamily of the M1 aminopeptidase family. NPEPPS encodes puromycin-sensitive aminopeptidase, a zinc metallopeptidase that hydrolyzes amino acids from the N-terminus of its substrate. The protein has 2 isoforms and is located in the cytoplasm.

实验方案

Product Specific Protocols
WB protocol for NPEPPS antibody 84230-5-RRDownload protocol
IHC protocol for NPEPPS antibody 84230-5-RRDownload protocol
IF protocol for NPEPPS antibody 84230-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols