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NPL Polyclonal antibody

NPL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16715-1-AP

Print datasheet

Synonyms

N-acetylneuraminate pyruvate-lyase, N-acetylneuraminate lyase, N acetylneuraminate lyase, EC:4.1.3.3, C1orf13

验证数据展示

  • WB analysis using 16715-1-AP

    WB analysis using 16715-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16715-1-AP (NPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 16715-1-AP

    IHC staining of human ovary cancer using 16715-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16715-1-AP (NPL antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 16715-1-AP

    IF Staining of MCF-7 using 16715-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NPL antibody (16715-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse kidney tissue, Raji cells, rat brain tissue, rat kidney tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16715-1-AP targets NPL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NPL fusion protein Ag10137 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase)
Synonyms N-acetylneuraminate pyruvate-lyase, N-acetylneuraminate lyase, N acetylneuraminate lyase, EC:4.1.3.3, C1orf13
Calculated Molecular Weight 27 kDa, 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC058003
Gene Symbol NPL
Gene ID (NCBI) 80896
RRIDAB_2154866
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BXD5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The N-acetylneuraminate lyase(NPL) from human (hNAL) which consists of 320 amino acids, has a molecular mass of 35.2 kDa and it catalyses the reversible aldol reaction of sialic acid, and has been extensively used in the synthesis of sialic acids and its analogues(PMID:19057931). The enzyme is widely distributed and are present in numerous pro- and eukaryotic cells, in which they are localized only in the cytosol(PMID:12453637). It belongs to the DHDPS family and has 5 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for NPL antibody 16715-1-APDownload protocol
IHC protocol for NPL antibody 16715-1-APDownload protocol
IF protocol for NPL antibody 16715-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols