验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, K-562 cells, LNCaP cells, MCF-7 cells, NIH/3T3 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11451-1-AP targets NQO1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, pig, rabbit, chicken, bovine, goat |
| 免疫原 | NQO1 fusion protein Ag2009 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | NAD(P)H dehydrogenase, quinone 1 |
| 别名 | DTD, DT diaphorase, DIA4, DIA 4, DHQU |
| 计算分子量 | 274 aa, 31 kDa |
| 观测分子量 | 31 kDa |
| GenBank蛋白编号 | BC007659 |
| 基因名称 | NQO1 |
| Gene ID (NCBI) | 1728 |
| RRID | AB_2298729 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P15559 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640; 18848868 ) This antibody recognizes all the three isoforms (26-27 kDa and 31 kDa) of NQO1 and the homo-dimer form (66-70 kDa) of NQO1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NQO1 antibody 11451-1-AP | Download protocol |
| IHC protocol for NQO1 antibody 11451-1-AP | Download protocol |
| IF protocol for NQO1 antibody 11451-1-AP | Download protocol |
| IP protocol for NQO1 antibody 11451-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature The CoQ oxidoreductase FSP1 acts parallel to GPX4 to inhibit ferroptosis.
| ||
Bioact Mater A two-pronged approach to inhibit ferroptosis of MSCs caused by the iron overload in postmenopausal osteoporosis and promote osseointegration of titanium implant | ||
Sci Transl Med Activation of NRF2 ameliorates oxidative stress and cystogenesis in autosomal dominant polycystic kidney disease. | ||
Nat Commun Microglia clear neuron-released α-synuclein via selective autophagy and prevent neurodegeneration. | ||
Nat Commun Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents. |