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验证数据展示

  • FC experiment of MCF-7 using CL555-11451

    FC experiment of MCF-7 using CL555-11451

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human NQO1 (CL555-11451) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of MCF-7 using CL555-11451

    IF Staining of MCF-7 using CL555-11451

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®555 NQO1 antibody (CL555-11451) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®555-conjugated NQO1 Polyclonal antibody

NQO1 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL555-11451

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

DTD, DT diaphorase, DIA4, DIA 4, DHQU

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®555
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 11451-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-11451 targets NQO1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 NQO1 fusion protein Ag2009 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NAD(P)H dehydrogenase, quinone 1
别名 DTD, DT diaphorase, DIA4, DIA 4, DHQU
计算分子量 274 aa, 31 kDa
观测分子量 31 kDa
GenBank蛋白编号BC007659
基因名称 NQO1
Gene ID (NCBI) 1728
RRIDAB_2919601
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP15559
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640; 18848868 ) This antibody recognizes all the three isoforms (26-27 kDa and 31 kDa) of NQO1 and the homo-dimer form (66-70 kDa) of NQO1.

实验方案

Product Specific Protocols
IF protocol for CL555 NQO1 antibody CL555-11451Download protocol
Standard Protocols
Click here to view our Standard Protocols