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验证数据展示

  • FC experiment of MCF-7 using CL647-67240

    FC experiment of MCF-7 using CL647-67240

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human NQO1 (CL647-67240, Clone:1E5G7) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 647-conjugated NQO1 Monoclonal antibody

NQO1 Monoclonal Antibody for FC (Intra)
Cat No. CL647-67240

产品说明书

CloneNo. 1E5G7

宿主/亚型

Mouse / IgG2a

种属反应性

human, rat, pig

应用

FC (Intra)

别名

DT diaphorase, DIA4, DIA 4, DHQU, Azoreductase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 67240-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-67240 targets NQO1 in FC (Intra) applications and shows reactivity with human, rat, pig samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, rat, pig
免疫原 NQO1 fusion protein Ag28933 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 NAD(P)H dehydrogenase, quinone 1
别名 DT diaphorase, DIA4, DIA 4, DHQU, Azoreductase
计算分子量 274 aa, 31 kDa
观测分子量 29-31 kDa
GenBank蛋白编号BC007659
基因名称 NQO1
Gene ID (NCBI) 1728
RRIDAB_2935076
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP15559
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640; 18848868 )

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 NQO1 antibody CL647-67240Download protocol
Standard Protocols
Click here to view our Standard Protocols