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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated NQO2 Polyclonal antibody

NQO2 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-15767

Print datasheet

Synonyms

NMOR2, EC:1.10.5.1

验证数据展示

  • IF Staining of HeLa using CL488-15767

    IF Staining of HeLa using CL488-15767

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 NQO2 antibody (CL488-15767) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 15767-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-15767 targets NQO2 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen NQO2 fusion protein Ag8403 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NAD(P)H dehydrogenase, quinone 2
Synonyms NMOR2, EC:1.10.5.1
Calculated Molecular Weight 231 aa, 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC006096
Gene Symbol NQO2
Gene ID (NCBI) 4835
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP16083
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NQO2, also named as QR2 and NMOR2, belongs to the NAD(P)H dehydrogenase (quinone) family. It serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. The cytosolic quinone oxidoreductases NQO1 and NQO2 protect cells against oxidative stress by detoxifying quinones and preventing redox cycling. NQO1 and NQO2 are important endogenous factors in regulation of immune response and autoimmunity.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NQO2 antibody CL488-15767Download protocol
Standard Protocols
Click here to view our Standard Protocols