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NR1H4 Recombinant antibody

NR1H4 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

4B4

Cat no : 81820-2-RR

Print datasheet

Synonyms

Farnesol receptor HRR-1, Farnesoid X-activated receptor, Farnesoid X activated receptor, Bile acid receptor, BAR

验证数据展示

  • WB analysis using 81820-2-RR

    WB analysis using 81820-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81820-2-RR (NR1H4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 81820-2-RR

    WB analysis of HepG2 using 81820-2-RR

    WB result of NR1H4 antibody (81820-2-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NR1H4 transfected HepG2 cells.

  • FC experiment of HepG2 using 81820-2-RR

    FC experiment of HepG2 using 81820-2-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NR1H4 Recombinant antibody (81820-2-RR, Clone:4B4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 81820-2-RR

    Affinity and Kinetic Characterization of 81820-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81820-2-RR against Human NR1H4 were performed. The affinity constant is 0.807 nM.

经过测试的应用

Positive WB detected inMKN-45 cells, HepG2 cells, HEK-293 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81820-2-RR targets NR1H4 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen NR1H4 fusion protein Ag21948 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name nuclear receptor subfamily 1, group H, member 4
Synonyms Farnesol receptor HRR-1, Farnesoid X-activated receptor, Farnesoid X activated receptor, Bile acid receptor, BAR
Calculated Molecular Weight 486 aa, 56 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC130573
Gene Symbol NR1H4
Gene ID (NCBI) 9971
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96RI1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nuclear Receptor subfamily 1, group H, member 4 (NR1H4, also known as FXR) is a receptor for bile acids and has an important role in regulating energy metabolism in liver, muscle and adipose tissues in humans and animals. NR1H4 is highly expressed in liver and has a role in lipid metabolism, whereas none of the other protein-coding genes in the region has known biological links with cholesterol, further supporting a role for NR1H4 in regulation of cholesterol levels. NR1H4 have four isoforms, i.e., FXRalpha1, FXRalpha2, FXRbeta1, and FXRbeta2, and each isoform has a different expressional level and transcriptional activity depending on its location within specific tissues. (PMID: 14733360, PMID: 30787420)

实验方案

Product Specific Protocols
WB protocol for NR1H4 antibody 81820-2-RRDownload protocol
FC protocol for NR1H4 antibody 81820-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols