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验证数据展示

  • WB analysis using 81820-2-RR

    WB analysis using 81820-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81820-2-RR (NR1H4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 81820-2-RR

    WB analysis of HepG2 using 81820-2-RR

    WB result of NR1H4 antibody (81820-2-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NR1H4 transfected HepG2 cells.

  • FC experiment of HepG2 using 81820-2-RR

    FC experiment of HepG2 using 81820-2-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NR1H4 Recombinant antibody (81820-2-RR, Clone:4B4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 81820-2-RR

    Affinity and Kinetic Characterization of 81820-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81820-2-RR against Human NR1H4 were performed. The affinity constant is 0.807 nM.

  • Featured Product
  • KD/KO验证

NR1H4 Recombinant antibody

NR1H4 Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 81820-2-RR

产品说明书

CloneNo. 4B4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

Farnesol receptor HRR-1, Farnesoid X-activated receptor, Farnesoid X activated receptor, Bile acid receptor, BAR

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMKN-45 cells, HepG2 cells, HEK-293 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81820-2-RR targets NR1H4 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 NR1H4 fusion protein Ag21948 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nuclear receptor subfamily 1, group H, member 4
别名 Farnesol receptor HRR-1, Farnesoid X-activated receptor, Farnesoid X activated receptor, Bile acid receptor, BAR
计算分子量 486 aa, 56 kDa
观测分子量 48 kDa
GenBank蛋白编号BC130573
基因名称 NR1H4
Gene ID (NCBI) 9971
RRIDAB_3670508
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96RI1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nuclear Receptor subfamily 1, group H, member 4 (NR1H4, also known as FXR) is a receptor for bile acids and has an important role in regulating energy metabolism in liver, muscle and adipose tissues in humans and animals. NR1H4 is highly expressed in liver and has a role in lipid metabolism, whereas none of the other protein-coding genes in the region has known biological links with cholesterol, further supporting a role for NR1H4 in regulation of cholesterol levels. NR1H4 have four isoforms, i.e., FXRalpha1, FXRalpha2, FXRbeta1, and FXRbeta2, and each isoform has a different expressional level and transcriptional activity depending on its location within specific tissues. (PMID: 14733360, PMID: 30787420)

实验方案

Product Specific Protocols
WB protocol for NR1H4 antibody 81820-2-RRDownload protocol
FC protocol for NR1H4 antibody 81820-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols