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NR3C2 Polyclonal antibody

NR3C2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 21854-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (2)

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

MR, MLR, MCR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse kidney tissue, HEK-293 cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy NR3C2 Ready-To-Use IHC Kit. NR3C2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21854-1-AP targets NR3C2 in WB, IHC, IF/ICC, IP, chIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, chIP
经测试反应性 human, mouse
文献引用反应性human, mouse, rat, monkey
免疫原 NR3C2 fusion protein Ag16410 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear receptor subfamily 3, group C, member 2
别名 MR, MLR, MCR
计算分子量 984 aa, 107 kDa
观测分子量 94-110 kDa
GenBank蛋白编号BC111758
基因名称 NR3C2
Gene ID (NCBI) 4306
RRIDAB_10858785
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08235
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nuclear receptor subfamily 3 group C member 2 (NR3C2), also known as Mineralocorticoid receptor (MCR or MR), is a member of the steroid/thyroid /retinoic nuclear hormone receptor superfamily that has been shown to activate gene transcription in response to aldosterone binding. Regulation of the mineralocorticoid receptors occurs through either receptor down-regulation (negative autoregulation) or hormone-mediated upregulation (positive autoregulation). MCR association with HSP 90 appears to be required for hormone binding to MCR and subsequent MCR activation.

实验方案

Product Specific Protocols
WB protocol for NR3C2 antibody 21854-1-APDownload protocol
IHC protocol for NR3C2 antibody 21854-1-APDownload protocol
IF protocol for NR3C2 antibody 21854-1-APDownload protocol
IP protocol for NR3C2 antibody 21854-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIHC

J Pineal Res

Melatonin prevents experimental central serous chorioretinopathy in rats.

Authors - Shanshan Yu
humanWB

EMBO Rep

High salt activates p97 to reduce host antiviral immunity by restricting Viperin induction.

Authors - Yukang Yuan
ratWB

Oncotarget

Impact of prenatal cold stress on placental physiology, inflammatory response, and apoptosis in rats.

Authors - Shuai Lian
ratIHC,IF

Int Immunopharmacol

UUO induces lung fibrosis with macrophage-myofibroblast transition in rats.

Authors - Fan Yang
ratWB

Behav Brain Res

Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats.

Authors - Shuai Lian
ratWB

Behav Brain Res

Prenatal cold stress: Effect on maternal hippocampus and offspring behavior in rats.

Authors - Shuai Lian
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