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验证数据展示

  • IF Staining of HepG2 using CL488-21854

    IF Staining of HepG2 using CL488-21854

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 NR3C2 antibody (CL488-21854) at dilution of 1:200, CL594-phalloidin (red).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated NR3C2 Polyclonal antibody

NR3C2 Polyclonal Antibody for IF/ICC
Cat No. CL488-21854

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC

别名

MR, MLR, MCR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy NR3C2 Ready-To-Use IHC Kit. NR3C2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 21854-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-21854 targets NR3C2 in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原 NR3C2 fusion protein Ag16410 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear receptor subfamily 3, group C, member 2
别名 MR, MLR, MCR
计算分子量 984 aa, 107 kDa
观测分子量 94-110 kDa
GenBank蛋白编号BC111758
基因名称 NR3C2
Gene ID (NCBI) 4306
RRIDAB_3084057
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08235
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nuclear receptor subfamily 3 group C member 2 (NR3C2), also known as Mineralocorticoid receptor (MCR or MR), is a member of the steroid/thyroid /retinoic nuclear hormone receptor superfamily that has been shown to activate gene transcription in response to aldosterone binding. Regulation of the mineralocorticoid receptors occurs through either receptor down-regulation (negative autoregulation) or hormone-mediated upregulation (positive autoregulation). MCR association with HSP 90 appears to be required for hormone binding to MCR and subsequent MCR activation.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NR3C2 antibody CL488-21854Download protocol
Standard Protocols
Click here to view our Standard Protocols