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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated NR3C2 Polyclonal antibody

NR3C2 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-21854

Print datasheet

Synonyms

MCR, Mineralocorticoid receptor, MLR, MR, NR3C2

验证数据展示

  • IF Staining of HepG2 using CL488-21854

    IF Staining of HepG2 using CL488-21854

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 NR3C2 antibody (CL488-21854) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy NR3C2 Ready-To-Use IHC Kit. NR3C2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 21854-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-21854 targets NR3C2 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen NR3C2 fusion protein Ag16410 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nuclear receptor subfamily 3, group C, member 2
Synonyms MCR, Mineralocorticoid receptor, MLR, MR, NR3C2
Calculated Molecular Weight 984 aa, 107 kDa
Observed Molecular Weight 94-110 kDa
GenBank Accession NumberBC111758
Gene Symbol NR3C2
Gene ID (NCBI) 4306
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08235
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nuclear receptor subfamily 3 group C member 2 (NR3C2), also known as Mineralocorticoid receptor (MCR or MR), is a member of the steroid/thyroid /retinoic nuclear hormone receptor superfamily that has been shown to activate gene transcription in response to aldosterone binding. Regulation of the mineralocorticoid receptors occurs through either receptor down-regulation (negative autoregulation) or hormone-mediated upregulation (positive autoregulation). MCR association with HSP 90 appears to be required for hormone binding to MCR and subsequent MCR activation.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NR3C2 antibody CL488-21854Download protocol
Standard Protocols
Click here to view our Standard Protocols