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NRBP2 Monoclonal antibody, PBS Only

NRBP2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4F10H7

Cat No : 66687-1-PBS

Print datasheet

Synonyms

DKFZp434P086, NRBP2, pp9320, TRG 16, TRG16

验证数据展示

  • WB analysis using 66687-1-Ig (same clone as 66687-1-PBS)

    WB analysis using 66687-1-Ig (same clone as 66687-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66687-1-Ig (NRBP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66687-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 66687-1-Ig (same clone as 66687-1-PBS)

    IF Staining of HEK-293 using 66687-1-Ig (same clone as 66687-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 66687-1-Ig (NRBP2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66687-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66687-1-Ig (same clone as 66687-1-PBS)

    IHC staining of mouse brain using 66687-1-Ig (same clone as 66687-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66687-1-Ig (NRBP2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66687-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66687-1-Ig (same clone as 66687-1-PBS)

    IHC staining of mouse brain using 66687-1-Ig (same clone as 66687-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66687-1-Ig (NRBP2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66687-1-PBS in a different storage buffer formulation.

产品信息

66687-1-PBS targets NRBP2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen NRBP2 fusion protein Ag17011 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name nuclear receptor binding protein 2
Synonyms DKFZp434P086, NRBP2, pp9320, TRG 16, TRG16
Calculated Molecular Weight 501 aa, 58 kDa
Observed Molecular Weight 58 kDa, 33 kDa
GenBank Accession NumberBC113873
Gene Symbol NRBP2
Gene ID (NCBI) 340371
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NSY0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nuclear receptor binding protein 2(NRBP2), also named as TRG16, belongs to the Ser/Thr protein kinase family. NRBP2 can regulate apoptosis of neural progenitor cells during their differentiation. Expression of NRBP2 was up-regulated following differentiation in rodent neural stem/progenitor cells. It has 4 isoforms with MW of 58, 33, 36 and 14 kDa. Endogenous NRBP2 in HEK293 cells has an apparent molecular mass of 55-60 kD by SDS-PAGE.