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验证数据展示

  • WB analysis using 66687-1-Ig

    WB analysis using 66687-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66687-1-Ig (NRBP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 66687-1-Ig

    IF Staining of HEK-293 using 66687-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 66687-1-Ig (NRBP2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IHC staining of mouse brain using 66687-1-Ig

    IHC staining of mouse brain using 66687-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66687-1-Ig (NRBP2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 66687-1-Ig

    IHC staining of mouse brain using 66687-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66687-1-Ig (NRBP2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NRBP2 Monoclonal antibody

NRBP2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66687-1-Ig

产品说明书

CloneNo. 4F10H7

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, Rat, Pig

应用

WB, IHC, IF/ICC, ELISA

别名

DKFZp434P086, NRBP2, pp9320, TRG 16, TRG16

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, pig brain tissue, A549 cells, HeLa cells, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66687-1-Ig targets NRBP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, Rat, Pig
免疫原 NRBP2 fusion protein Ag17011 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 nuclear receptor binding protein 2
别名 DKFZp434P086, NRBP2, pp9320, TRG 16, TRG16
计算分子量 501 aa, 58 kDa
观测分子量 58 kDa, 33 kDa
GenBank蛋白编号BC113873
基因名称 NRBP2
Gene ID (NCBI) 340371
RRIDAB_2882041
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NSY0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nuclear receptor binding protein 2(NRBP2), also named as TRG16, belongs to the Ser/Thr protein kinase family. NRBP2 can regulate apoptosis of neural progenitor cells during their differentiation. Expression of NRBP2 was up-regulated following differentiation in rodent neural stem/progenitor cells. It has 4 isoforms with MW of 58, 33, 36 and 14 kDa. Endogenous NRBP2 in HEK293 cells has an apparent molecular mass of 55-60 kD by SDS-PAGE.

实验方案

Product Specific Protocols
WB protocol for NRBP2 antibody 66687-1-IgDownload protocol
IHC protocol for NRBP2 antibody 66687-1-IgDownload protocol
IF protocol for NRBP2 antibody 66687-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols