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验证数据展示

  • IF Staining of HEK-293 using CL594-66687

    IF Staining of HEK-293 using CL594-66687

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite®594 NRBP2 antibody (CL594-66687, Clone: 4F10H7 ) at dilution of 1:200.

CoraLite®594-conjugated NRBP2 Monoclonal antibody

NRBP2 Monoclonal Antibody for IF/ICC
Cat No. CL594-66687

Print datasheet

CloneNo. 4F10H7

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

IF/ICC

Synonyms

DKFZp434P086, NRBP2, pp9320, TRG 16, TRG16

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHEK-293 cells, HeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66687-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66687 targets NRBP2 in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen NRBP2 fusion protein Ag17011 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name nuclear receptor binding protein 2
Synonyms DKFZp434P086, NRBP2, pp9320, TRG 16, TRG16
Calculated Molecular Weight 501 aa, 58 kDa
Observed Molecular Weight 58 kDa, 33 kDa
GenBank Accession NumberBC113873
Gene Symbol NRBP2
Gene ID (NCBI) 340371
RRIDAB_2883613
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NSY0
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nuclear receptor binding protein 2(NRBP2), also named as TRG16, belongs to the Ser/Thr protein kinase family. NRBP2 can regulate apoptosis of neural progenitor cells during their differentiation. Expression of NRBP2 was up-regulated following differentiation in rodent neural stem/progenitor cells. It has 4 isoforms with MW of 58, 33, 36 and 14 kDa. Endogenous NRBP2 in HEK293 cells has an apparent molecular mass of 55-60 kD by SDS-PAGE.

实验方案

Product Specific Protocols
IF protocol for CL594 NRBP2 antibody CL594-66687Download protocol
Standard Protocols
Click here to view our Standard Protocols