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NRCAM Monoclonal antibody, PBS Only

NRCAM Monoclonal Antibody for WB, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E1C3

Cat no : 68291-1-PBS

Print datasheet

Synonyms

hBravo, KIAA0343, Neuronal surface protein Bravo, Ng CAM related, Nr CAM, NRCAM

验证数据展示

  • WB analysis using 68291-1-Ig (same clone as 68291-1-PBS)

    WB analysis using 68291-1-Ig (same clone as 68291-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68291-1-Ig (NRCAM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68291-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 68291-1-Ig (same clone as 68291-1-PBS)

    IF Staining of mouse brain using 68291-1-Ig (same clone as 68291-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NRCAM antibody (68291-1-Ig, Clone: 2E1C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68291-1-PBS in a different storage buffer formulation.

产品信息

68291-1-PBS targets NRCAM in WB, IF-P, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB, IF-P, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen NRCAM fusion protein Ag20558 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name neuronal cell adhesion molecule
Synonyms hBravo, KIAA0343, Neuronal surface protein Bravo, Ng CAM related, Nr CAM, NRCAM
Calculated Molecular Weight 1304 aa, 144 kDa
Observed Molecular Weight 130-140 kDa
GenBank Accession NumberBC098401
Gene Symbol NRCAM
Gene ID (NCBI) 4897
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92823
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Neuronal cell adhesion molecule (NRCAM) is a member of the L1 subfamily of cell adhesion molecules (CAMs) that belong to the immunoglobulin superfamily (PMID: 11329126). NRCAM is a transmembrane protein composed of six Ig-like domains and five fibronectin type-III repeats in the extracellular region, with a highly conserved cytoplasmic tail. It is mainly expressed in the nervous system and is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. NRCAM is also expressed outside the nervous system. Altered expression of NRCAM has been associated with tumor progression in diverse organs (PMID: 22182708).