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验证数据展示

  • WB analysis using 68291-1-Ig

    WB analysis using 68291-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68291-1-Ig (NRCAM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 68291-1-Ig

    IF Staining of mouse brain using 68291-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NRCAM antibody (68291-1-Ig, Clone: 2E1C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

NRCAM Monoclonal antibody

NRCAM Monoclonal Antibody for WB, IF-P, ELISA
Cat No. 68291-1-Ig

产品说明书

CloneNo. 2E1C3

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse, Rat, Pig

应用

WB, IF-P, ELISA

别名

hBravo, KIAA0343, Neuronal surface protein Bravo, Ng CAM related, Nr CAM, NRCAM

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat brain tissue, pig brain tissue, rat cerebellum tissue, mouse brain tissue, pig cerebellum tissue
Positive IF-P detected inmouse brain tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68291-1-Ig targets NRCAM in WB, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 Human, Mouse, Rat, Pig
免疫原 NRCAM fusion protein Ag20558 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 neuronal cell adhesion molecule
别名 hBravo, KIAA0343, Neuronal surface protein Bravo, Ng CAM related, Nr CAM, NRCAM
计算分子量 1304 aa, 144 kDa
观测分子量 130-140 kDa
GenBank蛋白编号BC098401
基因名称 NRCAM
Gene ID (NCBI) 4897
RRIDAB_2935371
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92823
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuronal cell adhesion molecule (NRCAM) is a member of the L1 subfamily of cell adhesion molecules (CAMs) that belong to the immunoglobulin superfamily (PMID: 11329126). NRCAM is a transmembrane protein composed of six Ig-like domains and five fibronectin type-III repeats in the extracellular region, with a highly conserved cytoplasmic tail. It is mainly expressed in the nervous system and is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. NRCAM is also expressed outside the nervous system. Altered expression of NRCAM has been associated with tumor progression in diverse organs (PMID: 22182708).

实验方案

Product Specific Protocols
WB protocol for NRCAM antibody 68291-1-IgDownload protocol
IF protocol for NRCAM antibody 68291-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols