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验证数据展示

  • IF Staining of HEK-293 using CL488-68291

    IF Staining of HEK-293 using CL488-68291

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 NRCAM antibody (CL488-68291, Clone: 2E1C3 ) at dilution of 1:400.

CoraLite® Plus 488-conjugated NRCAM Monoclonal antibody

NRCAM Monoclonal Antibody for IF/ICC
Cat No. CL488-68291

产品说明书

CloneNo. 2E1C3

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse, Rat, Pig

应用

IF/ICC

别名

hBravo, KIAA0343, Neuronal surface protein Bravo, Ng CAM related, Nr CAM, NRCAM

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 68291-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68291 targets NRCAM in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Pig samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, Mouse, Rat, Pig
免疫原 NRCAM fusion protein Ag20558 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 neuronal cell adhesion molecule
别名 hBravo, KIAA0343, Neuronal surface protein Bravo, Ng CAM related, Nr CAM, NRCAM
计算分子量 1304 aa, 144 kDa
观测分子量 130-140 kDa
GenBank蛋白编号BC098401
基因名称 NRCAM
Gene ID (NCBI) 4897
RRIDAB_3672990
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92823
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuronal cell adhesion molecule (NRCAM) is a member of the L1 subfamily of cell adhesion molecules (CAMs) that belong to the immunoglobulin superfamily (PMID: 11329126). NRCAM is a transmembrane protein composed of six Ig-like domains and five fibronectin type-III repeats in the extracellular region, with a highly conserved cytoplasmic tail. It is mainly expressed in the nervous system and is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. NRCAM is also expressed outside the nervous system. Altered expression of NRCAM has been associated with tumor progression in diverse organs (PMID: 22182708).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NRCAM antibody CL488-68291Download protocol
Standard Protocols
Click here to view our Standard Protocols