• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

NRF1/nuclear respiratory factor 1 Recombinant antibody, PBS Only

NRF1/nuclear respiratory factor 1 Recombinant Antibody for WB, IF, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230394B1

Cat No : 83092-1-PBS

Print datasheet

Synonyms

ALPHA PAL, NRF 1, NRF1, nuclear respiratory factor 1

验证数据展示

  • WB analysis using 83092-1-RR (same clone as 83092-1-PBS)

    WB analysis using 83092-1-RR (same clone as 83092-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83092-1-RR (NRF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83092-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83092-1-RR (same clone as 83092-1-PBS)

    IF Staining of HepG2 using 83092-1-RR (same clone as 83092-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NRF1/nuclear respiratory factor 1 antibody (83092-1-RR, Clone: 230394B1 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83092-1-PBS in a different storage buffer formulation.

  • Affinity experiments of 83092-1-RR

    Affinity experiments of 83092-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83092-1-RR against Human NRF1/nuclear respiratory factor 1 were performed. The affinity constant is 0.115 nM.

Planning an IHC experiment? We recommend our IHCeasy NRF1 Ready-To-Use IHC Kit. NRF1 primary antibody included.

产品信息

83092-1-PBS targets NRF1/nuclear respiratory factor 1 in WB, IF, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IF, Indirect ELISA Application Description
Tested Reactivity Human, mouse
Immunogen NRF1/nuclear respiratory factor 1 fusion protein Ag3172 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name nuclear respiratory factor 1
Synonyms ALPHA PAL, NRF 1, NRF1, nuclear respiratory factor 1
Calculated Molecular Weight 522 aa, 56 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC016925
Gene Symbol NRF1
Gene ID (NCBI) 4899
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16656
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.