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NRF1/nuclear respiratory factor 1 Recombinant antibody

NRF1/nuclear respiratory factor 1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230394B1

Cat no : 83092-1-RR

Print datasheet

Synonyms

NRF1, nuclear respiratory factor 1, NRF 1, Alpha-pal, Alpha palindromic-binding protein

验证数据展示

  • WB analysis using 83092-1-RR

    WB analysis using 83092-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83092-1-RR (NRF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 83092-1-RR

    IF Staining of HepG2 using 83092-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NRF1/nuclear respiratory factor 1 antibody (83092-1-RR, Clone: 230394B1 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HEK-293T using 83092-1-RR

    FC experiment of HEK-293T using 83092-1-RR

    1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug Anti-Human NRF1 (83092-1-RR, Clone:230394B1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83092-1-RR

    Affinity and Kinetic Characterization of 83092-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83092-1-RR against Human NRF1/nuclear respiratory factor 1 were performed. The affinity constant is 0.115 nM.

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, RAW 264.7 cells, NIH/3T3 cells, U2OS cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy NRF1 Ready-To-Use IHC Kit. NRF1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83092-1-RR targets NRF1/nuclear respiratory factor 1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen NRF1/nuclear respiratory factor 1 fusion protein Ag3172 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name nuclear respiratory factor 1
Synonyms NRF1, nuclear respiratory factor 1, NRF 1, Alpha-pal, Alpha palindromic-binding protein
Calculated Molecular Weight 522 aa, 56 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC016925
Gene Symbol NRF1
Gene ID (NCBI) 4899
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16656
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.

实验方案

Product Specific Protocols
WB protocol for NRF1/nuclear respiratory factor 1 antibody 83092-1-RRDownload protocol
IF protocol for NRF1/nuclear respiratory factor 1 antibody 83092-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols