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验证数据展示

  • WB analysis using 80593-1-RR (same clone as 80593-1-PBS)

    WB analysis using 80593-1-RR (same clone as 80593-1-PBS)

    Untreated and MG132 treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80593-1-PBS in a different storage buffer formulation.

  • WB analysis using 80593-1-RR (same clone as 80593-1-PBS)

    WB analysis using 80593-1-RR (same clone as 80593-1-PBS)

    Untreated and MG132 treated HepG2 cells were subjected to SDS PAGE followed by western blot with 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80593-1-PBS in a different storage buffer formulation.

  • WB analysis using 80593-1-RR (same clone as 80593-1-PBS)

    WB analysis using 80593-1-RR (same clone as 80593-1-PBS)

    Untreated and MG132 treated HeLa cells were subjected to SDS PAGE followed by western blot with 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80593-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 80593-1-RR (same clone as 80593-1-PBS)

    IHC staining of human ovary tumor using 80593-1-RR (same clone as 80593-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80593-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFE2L2/NRF2 antibody included
  • FC experiment of MCF-7 using 80593-1-RR (same clone as 80593-1-PBS)

    FC experiment of MCF-7 using 80593-1-RR (same clone as 80593-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human NRF2, NFE2L2 (80593-1-RR, Clone:1I21) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80593-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 80593-1-RR (same clone as 80593-1-PBS)

    WB analysis of HeLa using 80593-1-RR (same clone as 80593-1-PBS)

    WB result of NRF2, NFE2L2 antibody (80593-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NRF2, NFE2L2 transfected HeLa cells. This data was developed using the same antibody clone with 80593-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 80593-1-RR (same clone as 80593-1-PBS)

    IP experiment of HeLa using 80593-1-RR (same clone as 80593-1-PBS)

    IP result of anti-NRF2, NFE2L2 (IP:80593-1-RR, 4ug; Detection:80593-1-RR 1:700) with HeLa cells lysate 1520 ug. This data was developed using the same antibody clone with 80593-1-PBS in a different storage buffer formulation.

  • IHC staining of Human bowens disease using 80593-1-RR (same clone as 80593-1-PBS)

    IHC staining of Human bowens disease using 80593-1-RR (same clone as 80593-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Brown disease slide using 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80593-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFE2L2/NRF2 antibody included
  • IHC staining of human ovary tumor using 80593-1-RR (same clone as 80593-1-PBS)

    IHC staining of human ovary tumor using 80593-1-RR (same clone as 80593-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80593-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFE2L2/NRF2 antibody included
  • IHC staining of human pancreas cancer using 80593-1-RR (same clone as 80593-1-PBS)

    IHC staining of human pancreas cancer using 80593-1-RR (same clone as 80593-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80593-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFE2L2/NRF2 antibody included
  • IHC staining of human pancreas cancer using 80593-1-RR (same clone as 80593-1-PBS)

    IHC staining of human pancreas cancer using 80593-1-RR (same clone as 80593-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80593-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFE2L2/NRF2 antibody included
  • IF Staining of HepG2 using 80593-1-RR (same clone as 80593-1-PBS)

    IF Staining of HepG2 using 80593-1-RR (same clone as 80593-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MG132 treated HepG2 cells using NRF2, NFE2L2 antibody (80593-1-RR, Clone: 1I21 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80593-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80593-1-RR

    Affinity and Kinetic Characterization of 80593-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80593-1-RR against Human NRF2, NFE2L2 were performed. The affinity constant is 0.191 nM.

  • Featured Product
  • KD/KO验证

NRF2, NFE2L2 Recombinant antibody, PBS Only

NRF2, NFE2L2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 80593-1-PBS

产品说明书

CloneNo. 1I21

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

NFE2L2, NRF2, HEBP1, NF-E2-related factor 2, NFE2-related factor 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy NFE2L2/NRF2 Ready-To-Use IHC Kit. NFE2L2/NRF2 primary antibody included.

产品信息

80593-1-PBS targets NRF2, NFE2L2 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 NRF2, NFE2L2 fusion protein Ag9489 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nuclear factor (erythroid-derived 2)-like 2
别名 NFE2L2, NRF2, HEBP1, NF-E2-related factor 2, NFE2-related factor 2
计算分子量 605 aa, 68 kDa
观测分子量 110 kDa, 68 kDa
GenBank蛋白编号BC011558
基因名称 NRF2
Gene ID (NCBI) 4780
RRIDAB_2918904
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16236
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress (PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC. As new investigators break into the emerging field of Nrf2 research, confusion regarding the correct migratory pattern of Nrf2 is causing doubts about the accuracy and reproducibility of published results. This letter provides solid evidence that the actually observed molecular weight of Nrf2 is about 70kDa and 95-110 kDa. (PMID: 22703241).