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  • Featured Product
  • KD/KO Validated

NRF2, NFE2L2 Recombinant antibody, PBS Only

NRF2, NFE2L2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1I21

Cat no : 80593-1-PBS

Print datasheet

Synonyms

NRF2, NFE2L2, HEBP1, Nrf 2, NFE2-related factor 2

验证数据展示

  • WB analysis using 80593-1-RR (same clone as 80593-1-PBS)

    WB analysis using 80593-1-RR (same clone as 80593-1-PBS)

    Untreated and MG132 treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80593-1-PBS in a different storage buffer formulation.

  • WB analysis using 80593-1-RR (same clone as 80593-1-PBS)

    WB analysis using 80593-1-RR (same clone as 80593-1-PBS)

    Untreated and MG132 treated HepG2 cells were subjected to SDS PAGE followed by western blot with 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80593-1-PBS in a different storage buffer formulation.

  • WB analysis using 80593-1-RR (same clone as 80593-1-PBS)

    WB analysis using 80593-1-RR (same clone as 80593-1-PBS)

    Untreated and MG132 treated HeLa cells were subjected to SDS PAGE followed by western blot with 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80593-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 80593-1-RR (same clone as 80593-1-PBS)

    IHC staining of human ovary tumor using 80593-1-RR (same clone as 80593-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80593-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFE2L2/NRF2 antibody included
  • FC experiment of MCF-7 using 80593-1-RR (same clone as 80593-1-PBS)

    FC experiment of MCF-7 using 80593-1-RR (same clone as 80593-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human NRF2, NFE2L2 (80593-1-RR, Clone:1I21) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80593-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 80593-1-RR (same clone as 80593-1-PBS)

    IP experiment of HeLa using 80593-1-RR (same clone as 80593-1-PBS)

    IP result of anti-NRF2, NFE2L2 (IP:80593-1-RR, 4ug; Detection:80593-1-RR 1:700) with HeLa cells lysate 1520 ug. This data was developed using the same antibody clone with 80593-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 80593-1-RR (same clone as 80593-1-PBS)

    IHC staining of human ovary tumor using 80593-1-RR (same clone as 80593-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80593-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFE2L2/NRF2 antibody included
  • IHC staining of human pancreas cancer using 80593-1-RR (same clone as 80593-1-PBS)

    IHC staining of human pancreas cancer using 80593-1-RR (same clone as 80593-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80593-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFE2L2/NRF2 antibody included
  • IHC staining of human pancreas cancer using 80593-1-RR (same clone as 80593-1-PBS)

    IHC staining of human pancreas cancer using 80593-1-RR (same clone as 80593-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80593-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFE2L2/NRF2 antibody included
  • IF Staining of HepG2 using 80593-1-RR (same clone as 80593-1-PBS)

    IF Staining of HepG2 using 80593-1-RR (same clone as 80593-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MG132 treated HepG2 cells using NRF2, NFE2L2 antibody (80593-1-RR, Clone: 1I21 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80593-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80593-1-RR

    Affinity and Kinetic Characterization of 80593-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80593-1-RR against Human NRF2, NFE2L2 were performed. The affinity constant is 0.191 nM.

Planning an IHC experiment? We recommend our IHCeasy NFE2L2/NRF2 Ready-To-Use IHC Kit. NFE2L2/NRF2 primary antibody included.

产品信息

80593-1-PBS targets NRF2, NFE2L2 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen NRF2, NFE2L2 fusion protein Ag9489 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name nuclear factor (erythroid-derived 2)-like 2
Synonyms NRF2, NFE2L2, HEBP1, Nrf 2, NFE2-related factor 2
Calculated Molecular Weight 605 aa, 68 kDa
Observed Molecular Weight110 kDa
GenBank Accession NumberBC011558
Gene Symbol NRF2
Gene ID (NCBI) 4780
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16236
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress (PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC. As new investigators break into the emerging field of Nrf2 research, confusion regarding the correct migratory pattern of Nrf2 is causing doubts about the accuracy and reproducibility of published results. This letter provides solid evidence that the actually observed molecular weight of Nrf2 is about 70kDa and 95-110 kDa. (PMID: 22703241).