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验证数据展示

  • FC experiment of HeLa using CL647-11345

    FC experiment of HeLa using CL647-11345

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human NSD3 (CL647-11345) (red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA .

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated NSD3 Polyclonal antibody

NSD3 Polyclonal Antibody for FC (Intra)
Cat No. CL647-11345

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

FC (Intra)

Synonyms

DKFZp667H044, FLJ20353, NSD3, pp14328, Protein whistle, WHSC1 like protein 1, WHSC1L1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 11345-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-11345 targets NSD3 in FC (Intra) applications and shows reactivity with human, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, rat
Immunogen NSD3 fusion protein Ag1894 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Wolf-Hirschhorn syndrome candidate 1-like 1
Synonyms DKFZp667H044, FLJ20353, NSD3, pp14328, Protein whistle, WHSC1 like protein 1, WHSC1L1
Calculated Molecular Weight 1437 aa, 162 kDa
Observed Molecular Weight 160-170 kDa, 68-72 kDa
GenBank Accession NumberBC012059
Gene Symbol WHSC1L1
Gene ID (NCBI) 54904
RRIDAB_2934855
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BZ95
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WHSC1L1, also named as NSD3 and DC28, belongs to the histone-lysine methyltransferase family and SET2 subfamily. It is histone methyltransferase. WHSC1L1 is a potently transforming oncogenes based on the number of altered phenotypes expressed by the cells. It contains a PWWP-domain that is a methyl-lysine recognition motif involved in histone code modification and epigenetic regulation of gene expression. Knockdown of WHSC1L1 in 8p11-12-amplified breast cancer cells resulted in profound loss of growth and survival of these cells. (PMID: 20940404). This antibody recognize the long isoform (160-170 kDa) and short isoform (68-72 kDa).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 NSD3 antibody CL647-11345Download protocol
Standard Protocols
Click here to view our Standard Protocols