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验证数据展示

  • IF Staining of A431 using CL647-68182

    IF Staining of A431 using CL647-68182

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 647 NSFL1C antibody (CL647-68182, Clone: 1F12E12 ) at dilution of 1:200.

  • FC experiment of A431 using CL647-68182

    FC experiment of A431 using CL647-68182

    1x10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated NSFL1C Monoclonal antibody (CL647-68182, Clone:1F12E12)(red), or 0.4 ug CoraLite® Plus 647 Mouse IgG1 Isotype Control (1F8D3) (CL647-66360-1, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 647-conjugated NSFL1C Monoclonal antibody

NSFL1C Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL647-68182

Print datasheet

CloneNo. 1F12E12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Synonyms

UBXN2C, UBX domain-containing protein 2C, p97 cofactor p47, NSFL1 cofactor p47, 1F12E12

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 68182-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-68182 targets NSFL1C in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen NSFL1C fusion protein Ag8040 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name NSFL1 (p97) cofactor (p47)
Synonyms UBXN2C, UBX domain-containing protein 2C, p97 cofactor p47, NSFL1 cofactor p47, 1F12E12
Calculated Molecular Weight 41 kDa
Observed Molecular Weight41 kDa
GenBank Accession NumberBC002801
Gene Symbol NSFL1C
Gene ID (NCBI) 55968
RRIDAB_3673647
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UNZ2
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 NSFL1C antibody CL647-68182Download protocol
FC protocol for CL Plus 647 NSFL1C antibody CL647-68182Download protocol
Standard Protocols
Click here to view our Standard Protocols