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CoraLite® Plus 647-conjugated NSFL1C Monoclonal antibody

NSFL1C Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1F12E12

Cat no : CL647-68182

Print datasheet

Synonyms

UBXN2C, UBX domain-containing protein 2C, p97 cofactor p47, NSFL1 cofactor p47, 1F12E12

验证数据展示

  • IF Staining of A431 using CL647-68182

    IF Staining of A431 using CL647-68182

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 647 NSFL1C antibody (CL647-68182, Clone: 1F12E12 ) at dilution of 1:200.

  • FC experiment of A431 using CL647-68182

    FC experiment of A431 using CL647-68182

    1x10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated NSFL1C Monoclonal antibody (CL647-68182, Clone:1F12E12)(red), or 0.4 ug CoraLite® Plus 647 Mouse IgG1 Isotype Control (1F8D3) (CL647-66360-1, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 68182-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-68182 targets NSFL1C in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen NSFL1C fusion protein Ag8040 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name NSFL1 (p97) cofactor (p47)
Synonyms UBXN2C, UBX domain-containing protein 2C, p97 cofactor p47, NSFL1 cofactor p47, 1F12E12
Calculated Molecular Weight 41 kDa
Observed Molecular Weight41 kDa
GenBank Accession NumberBC002801
Gene Symbol NSFL1C
Gene ID (NCBI) 55968
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UNZ2
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 NSFL1C antibody CL647-68182Download protocol
FC protocol for CL Plus 647 NSFL1C antibody CL647-68182Download protocol
Standard Protocols
Click here to view our Standard Protocols