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  • Featured Product
  • KD/KO Validated

NSUN2 Monoclonal antibody, PBS Only

NSUN2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H5A2

Cat no : 66580-1-PBS

Print datasheet

Synonyms

hTrm4, MISU, NSUN2, SAKI, TRM4

验证数据展示

  • WB analysis of COLO 320 using 66580-1-Ig (same clone as 66580-1-PBS)

    WB analysis of COLO 320 using 66580-1-Ig (same clone as 66580-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66580-1-Ig (NSUN2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

  • IHC staining of human oesophagus cancer using 66580-1-Ig (same clone as 66580-1-PBS)

    IHC staining of human oesophagus cancer using 66580-1-Ig (same clone as 66580-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66580-1-Ig (NSUN2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NSUN2 antibody included
  • WB analysis of HepG2 using 66580-1-Ig (same clone as 66580-1-PBS)

    WB analysis of HepG2 using 66580-1-Ig (same clone as 66580-1-PBS)

    WB result of NSUN2 antibody (66580-1-Ig; 1:40000; room temperature for 1.5 hours) with negative control and NSUN2 overexpressed HepG2 cells. This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66580-1-Ig (same clone as 66580-1-PBS)

    IF Staining of HepG2 using 66580-1-Ig (same clone as 66580-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66580-1-Ig (NSUN2 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 66580-1-Ig (same clone as 66580-1-PBS)

    IP experiment of HepG2 using 66580-1-Ig (same clone as 66580-1-PBS)

    IP result of anti-NSUN2 (IP:66580-1-Ig, 4ug; Detection:66580-1-Ig 1:4000) with HepG2 cells lysate 1920 ug. This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy NSUN2 Ready-To-Use IHC Kit. NSUN2 primary antibody included.

产品信息

66580-1-PBS targets NSUN2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen NSUN2 fusion protein Ag14791 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name NOL1/NOP2/Sun domain family, member 2
Synonyms hTrm4, MISU, NSUN2, SAKI, TRM4
Calculated Molecular Weight 767 aa, 86 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC001041
Gene Symbol NSUN2
Gene ID (NCBI) 54888
RRIDAB_2881940
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ08J23
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C