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  • Featured Product
  • KD/KO Validated

NSUN2 Monoclonal antibody, PBS Only

NSUN2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H5A2

Cat no : 66580-1-PBS

Print datasheet

Synonyms

hTrm4, MISU, NSUN2, SAKI, TRM4

验证数据展示

  • WB analysis of COLO 320 using 66580-1-Ig (same clone as 66580-1-PBS)

    WB analysis of COLO 320 using 66580-1-Ig (same clone as 66580-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66580-1-Ig (NSUN2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

  • IHC staining of human oesophagus cancer using 66580-1-Ig (same clone as 66580-1-PBS)

    IHC staining of human oesophagus cancer using 66580-1-Ig (same clone as 66580-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66580-1-Ig (NSUN2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NSUN2 antibody included
  • WB analysis of HepG2 using 66580-1-Ig (same clone as 66580-1-PBS)

    WB analysis of HepG2 using 66580-1-Ig (same clone as 66580-1-PBS)

    WB result of NSUN2 antibody (66580-1-Ig; 1:40000; room temperature for 1.5 hours) with negative control and NSUN2 overexpressed HepG2 cells. This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66580-1-Ig (same clone as 66580-1-PBS)

    IF Staining of HepG2 using 66580-1-Ig (same clone as 66580-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66580-1-Ig (NSUN2 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 66580-1-Ig (same clone as 66580-1-PBS)

    IP experiment of HepG2 using 66580-1-Ig (same clone as 66580-1-PBS)

    IP result of anti-NSUN2 (IP:66580-1-Ig, 4ug; Detection:66580-1-Ig 1:4000) with HepG2 cells lysate 1920 ug. This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy NSUN2 Ready-To-Use IHC Kit. NSUN2 primary antibody included.

产品信息

66580-1-PBS targets NSUN2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen NSUN2 fusion protein Ag14791 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name NOL1/NOP2/Sun domain family, member 2
Synonyms hTrm4, MISU, NSUN2, SAKI, TRM4
Calculated Molecular Weight 767 aa, 86 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC001041
Gene Symbol NSUN2
Gene ID (NCBI) 54888
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ08J23
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C