验证数据展示
经过测试的应用
Positive WB detected in | COLO 320 cells, HepG2 cells, Caco-2 cells, HEK-293 cells, Jurkat cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human oesophagus cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66580-1-Ig targets NSUN2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with Human samples.
经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB, IF, CoIP |
经测试反应性 | Human |
文献引用反应性 | human |
免疫原 | NSUN2 fusion protein Ag14791 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | NOL1/NOP2/Sun domain family, member 2 |
别名 | hTrm4, MISU, NSUN2, SAKI, TRM4 |
计算分子量 | 767 aa, 86 kDa |
观测分子量 | 100 kDa |
GenBank蛋白编号 | BC001041 |
基因名称 | NSUN2 |
Gene ID (NCBI) | 54888 |
RRID | AB_2881940 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q08J23 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for NSUN2 antibody 66580-1-Ig | Download protocol |
IHC protocol for NSUN2 antibody 66580-1-Ig | Download protocol |
IF protocol for NSUN2 antibody 66580-1-Ig | Download protocol |
IP protocol for NSUN2 antibody 66580-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun N6-methyladenosine modification of circNSUN2 facilitates cytoplasmic export and stabilizes HMGA2 to promote colorectal liver metastasis. | ||
Acta Neuropathol RNA methyltransferase NSun2 deficiency promotes neurodegeneration through epitranscriptomic regulation of tau phosphorylation | ||
Cell Metab NSUN2 is a glucose sensor suppressing cGAS/STING to maintain tumorigenesis and immunotherapy resistance | ||