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NSUN2 Monoclonal antibody

NSUN2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 66580-1-Ig

产品说明书

CloneNo. 2H5A2

宿主/亚型

Mouse / IgG2a

种属反应性

Human and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

hTrm4, MISU, NSUN2, SAKI, TRM4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inCOLO 320 cells, HepG2 cells, Caco-2 cells, HEK-293 cells, Jurkat cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman oesophagus cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy NSUN2 Ready-To-Use IHC Kit. NSUN2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66580-1-Ig targets NSUN2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF, CoIP
经测试反应性 Human
文献引用反应性human
免疫原 NSUN2 fusion protein Ag14791 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 NOL1/NOP2/Sun domain family, member 2
别名 hTrm4, MISU, NSUN2, SAKI, TRM4
计算分子量 767 aa, 86 kDa
观测分子量 100 kDa
GenBank蛋白编号BC001041
基因名称 NSUN2
Gene ID (NCBI) 54888
RRIDAB_2881940
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ08J23
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NSUN2 antibody 66580-1-IgDownload protocol
IHC protocol for NSUN2 antibody 66580-1-IgDownload protocol
IF protocol for NSUN2 antibody 66580-1-IgDownload protocol
IP protocol for NSUN2 antibody 66580-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

N6-methyladenosine modification of circNSUN2 facilitates cytoplasmic export and stabilizes HMGA2 to promote colorectal liver metastasis.

Authors - Ri-Xin Chen
human,IF

Acta Neuropathol

RNA methyltransferase NSun2 deficiency promotes neurodegeneration through epitranscriptomic regulation of tau phosphorylation

Authors - Yoon A Kim
humanWB,CoIP

Cell Metab

NSUN2 is a glucose sensor suppressing cGAS/STING to maintain tumorigenesis and immunotherapy resistance

Authors - Tingjin Chen
humanWB

Virol Sin

NSUN2 mediates distinct pathways to regulate enterovirus 71 replication

Authors - Lishi Liu
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