验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy NSUN2 Ready-To-Use IHC Kit. NSUN2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66580-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL488-66580 targets NSUN2 in IF/ICC applications and shows reactivity with Human samples.
| Tested Applications | IF/ICC Application Description |
| Cited Applications | IF |
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Immunogen | NSUN2 fusion protein Ag14791 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | NOL1/NOP2/Sun domain family, member 2 |
| Synonyms | FLJ20303, hTrm4, MISU, NSUN2, SAKI, TRM4 |
| Calculated Molecular Weight | 767 aa, 86 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC001041 |
| Gene Symbol | NSUN2 |
| Gene ID (NCBI) | 54888 |
| RRID | AB_2883358 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q08J23 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 NSUN2 antibody CL488-66580 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |