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CoraLite® Plus 488-conjugated NSUN4 Recombinant antibody

NSUN4 Uni-rAbTM Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

241115G11

Cat no : CL488-84275-3

Print datasheet

Synonyms

验证数据展示

  • IF Staining of HepG2 using CL488-84275-3

    IF Staining of HepG2 using CL488-84275-3

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 NSUN4 antibody (CL488-84275-3, Clone: 241115G11 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 84275-3-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL488-84275-3 is NSUN4 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen NSUN4 fusion protein Ag31828 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name NOL1/NOP2/Sun domain family, member 4
Synonyms
Calculated Molecular Weight 384 aa, 43 kDa
GenBank Accession NumberBC014441
Gene Symbol NSUN4
Gene ID (NCBI) 387338
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96CB9
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NSUN4 belongs to a family of RNA 5-methylcytosine (m5C) methyltransferases (MTases). MTERF4 recruits NSUN4 to the large ribosomal subunit in a process crucial for mitochondrial ribosome biogenesis. NSUN4 is a dual function protein, which on the one hand is needed for 12S rRNA methylation, and on the other hand interacts with MTERF4 to facilitate mitoribosomal assembly.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NSUN4 antibody CL488-84275-3Download protocol
Standard Protocols
Click here to view our Standard Protocols