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验证数据展示

  • WB analysis of HepG2 using 67789-1-Ig

    WB analysis of HepG2 using 67789-1-Ig

    WB result of NT5E/CD73 antibody (67789-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NT5E,CD73 transfected HepG2 cells.

  • WB analysis using 67789-1-Ig

    WB analysis using 67789-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67789-1-Ig (NT5E/CD73 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 67789-1-Ig

    IHC staining of human tonsillitis using 67789-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67789-1-Ig (NT5E/CD73 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 67789-1-Ig

    IHC staining of human tonsillitis using 67789-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67789-1-Ig (NT5E/CD73 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human appendicitis using 67789-1-Ig

    IF Staining of human appendicitis using 67789-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using NT5E/CD73 antibody (67789-1-Ig, Clone: 1G5G8 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human appendicitis using 67789-1-Ig

    IF Staining of human appendicitis using 67789-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using NT5E/CD73 antibody (67789-1-Ig, Clone: 1G5G8 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

CD73 Monoclonal antibody

CD73 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67789-1-Ig

产品说明书

CloneNo. 1G5G8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

NT5E, 5'-NT, 5'-deoxynucleotidase, 5' nucleotidase, ecto (CD73), 5 nucleotidase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, A549 cells, HT-29 cells, U-87 MG cells
Positive IHC detected inhuman tonsillitis tissue, mouse kidney tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman appendicitis tissue, human tonsillitis tissue, rat liver tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67789-1-Ig targets CD73 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CD73 fusion protein Ag30616 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 5'-nucleotidase, ecto (CD73)
别名 NT5E, 5'-NT, 5'-deoxynucleotidase, 5' nucleotidase, ecto (CD73), 5 nucleotidase
计算分子量 29 kDa, 63 kDa
观测分子量60-70 kDa
GenBank蛋白编号BC015940
基因名称 CD73
Gene ID (NCBI) 4907
RRIDAB_2918553
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP21589
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).

实验方案

Product Specific Protocols
WB protocol for CD73 antibody 67789-1-IgDownload protocol
IHC protocol for CD73 antibody 67789-1-IgDownload protocol
IF protocol for CD73 antibody 67789-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols