• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of human appendicitis using CL594-67789

    IF Staining of human appendicitis using CL594-67789

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using CoraLite®594 NT5E/CD73 antibody (CL594-67789, Clone: 1G5G8 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human appendicitis using CL594-67789

    IF Staining of human appendicitis using CL594-67789

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using CoraLite®594 NT5E/CD73 antibody (CL594-67789, Clone: 1G5G8 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated CD73 Monoclonal antibody

CD73 Monoclonal Antibody for IF-P
Cat No. CL594-67789

产品说明书

CloneNo. 1G5G8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF-P

别名

NT5E, 5'-NT, 5'-deoxynucleotidase, 5' nucleotidase, ecto (CD73), 5 nucleotidase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF-P detected inhuman appendicitis tissue, human tonsillitis tissue
For other applications, we recommend the unconjugated version of this antibody, 67789-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67789 targets CD73 in IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat
免疫原 CD73 fusion protein Ag30616 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 5'-nucleotidase, ecto (CD73)
别名 NT5E, 5'-NT, 5'-deoxynucleotidase, 5' nucleotidase, ecto (CD73), 5 nucleotidase
计算分子量 29 kDa, 63 kDa
观测分子量60-70 kDa
GenBank蛋白编号BC015940
基因名称 CD73
Gene ID (NCBI) 4907
RRIDAB_2920183
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP21589
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).

实验方案

Product Specific Protocols
IF protocol for CL594 CD73 antibody CL594-67789Download protocol
Standard Protocols
Click here to view our Standard Protocols