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CoraLite®594-conjugated NT5E/CD73 Monoclonal antibody

NT5E/CD73 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G5G8

Cat No : CL594-67789

Print datasheet

Synonyms

5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), CD73, E5NT, Ecto 5 nucleotidase, eN, eNT, NT, NT5, NT5E, NT5E,CD73, NTE

验证数据展示

  • IF Staining of human appendicitis using CL594-67789

    IF Staining of human appendicitis using CL594-67789

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using CoraLite®594 NT5E/CD73 antibody (CL594-67789, Clone: 1G5G8 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human appendicitis using CL594-67789

    IF Staining of human appendicitis using CL594-67789

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using CoraLite®594 NT5E/CD73 antibody (CL594-67789, Clone: 1G5G8 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IF detected inhuman appendicitis tissue, human tonsillitis tissue
For other applications, we recommend the unconjugated version of this antibody, 67789-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67789 targets NT5E/CD73 in IF applications and shows reactivity with Human, Rat, Mouse samples.

Tested Applications IF-P Application Description
Tested Reactivity Human, Rat, Mouse
Immunogen NT5E/CD73 fusion protein Ag30616 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name 5'-nucleotidase, ecto (CD73)
Synonyms 5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), CD73, E5NT, Ecto 5 nucleotidase, eN, eNT, NT, NT5, NT5E, NT5E,CD73, NTE
Calculated Molecular Weight 29 kDa, 63 kDa
Observed Molecular Weight60-70 kDa
GenBank Accession NumberBC015940
Gene Symbol NT5E
Gene ID (NCBI) 4907
RRIDAB_2920183
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP21589
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).

实验方案

Product Specific Protocols
IF protocol for CL594 NT5E/CD73 antibody CL594-67789Download protocol
Standard Protocols
Click here to view our Standard Protocols