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CoraLite® Plus 488-conjugated NTF2 Monoclonal antibody
NTF2 Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
4H7F9
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 66063-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of CL488-66063 is NTF2 Fusion Protein expressed in E. coli.
| Tested Applications | IF Application Description |
| Tested Reactivity | human |
| Immunogen | NTF2 fusion protein Ag7879 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | nuclear transport factor 2 |
| Synonyms | NTF 2, NTF2, nuclear transport factor 2, NUTF2, Placental protein 15, PP15 |
| Calculated Molecular Weight | 14 kDa |
| Observed Molecular Weight | 13 kDa |
| GenBank Accession Number | BC002348 |
| Gene Symbol | NTF2 |
| Gene ID (NCBI) | 10204 |
| RRID | AB_2883246 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P61970 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NUTF2 (nuclear transport factor 2), also named as NTF2, is a cytosolic protein responsible for nuclear import of Ran, a small Ras-like GTPase involved in a number of critical cellular processes, including cell cycle regulation, chromatin organization during mitosis, reformation of the nuclear envelope following mitosis, and controlling the directionality of nucleocytoplasmic transport. Nucleocytoplasmic translocation of NTF2 is regulated in mammalian cells, and may involve a tyrosine and/or threonine kinase-dependent signal transduction mechanism(s) (PMID: 22880006). The MW of this protein is 14 kDa, and this antibody specially recognises the 14 kDa protein.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 NTF2 antibody CL488-66063 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
