验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, mouse brain tissue, rat brain tissue, rat cerebellum tissue, rat kidney tissue |
| Positive IF/ICC detected in | PC-3 cells, SH-SY5Y cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12297-1-AP targets Neurotrophin 4 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC, CoIP, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | Neurotrophin 4 fusion protein Ag2938 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | neurotrophin 4 |
| Synonyms | NTF4, NT-4, NT4, NT 4, Neutrophic factor 4 |
| Calculated Molecular Weight | 210 aa, 22 kDa |
| Observed Molecular Weight | 70-80 kDa |
| GenBank Accession Number | BC012421 |
| Gene Symbol | Neurotrophin 4 |
| Gene ID (NCBI) | 4909 |
| RRID | AB_10598309 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P34130 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Neurotrophin 4 (NT-4) belongs to the family of neurotrophic factors, and it interacts with the tyrosine kinase B (trkB) receptor. NT-4 has neuroprotective effects following cerebral ischemia. Its role might be similar to brain-derived neurotrophic factor (BDNF) because both interact with trkB. Exercise also improves neural function by increasing neurotrophic factors. NT-4 exists in two forms, either as a dimer (80 kDa) or as a monomer (40-47 kDa) (PMID: 23526925).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Neurotrophin 4 antibody 12297-1-AP | Download protocol |
| IF protocol for Neurotrophin 4 antibody 12297-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Oncol Upregulated NTF4 in colorectal cancer promotes tumor development via regulating autophagy.
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