验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, mouse brain tissue, rat brain tissue, rat cerebellum tissue, rat kidney tissue |
Positive IF/ICC detected in | PC-3 cells, SH-SY5Y cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:3000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12297-1-AP targets Neurotrophin 4 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IHC, CoIP, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human |
免疫原 | Neurotrophin 4 fusion protein Ag2938 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | neurotrophin 4 |
别名 | NTF4, NT-4, NT4, NT 4, Neutrophic factor 4 |
计算分子量 | 210 aa, 22 kDa |
观测分子量 | 70-80 kDa |
GenBank蛋白编号 | BC012421 |
基因名称 | Neurotrophin 4 |
Gene ID (NCBI) | 4909 |
RRID | AB_10598309 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P34130 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Neurotrophin 4 (NT-4) belongs to the family of neurotrophic factors, and it interacts with the tyrosine kinase B (trkB) receptor. NT-4 has neuroprotective effects following cerebral ischemia. Its role might be similar to brain-derived neurotrophic factor (BDNF) because both interact with trkB. Exercise also improves neural function by increasing neurotrophic factors. NT-4 exists in two forms, either as a dimer (80 kDa) or as a monomer (40-47 kDa) (PMID: 23526925).
实验方案
Product Specific Protocols | |
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WB protocol for Neurotrophin 4 antibody 12297-1-AP | Download protocol |
IF protocol for Neurotrophin 4 antibody 12297-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Int J Oncol Upregulated NTF4 in colorectal cancer promotes tumor development via regulating autophagy.
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