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验证数据展示

  • IF Staining of PC-3 using CL488-12297

    IF Staining of PC-3 using CL488-12297

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using CoraLite® Plus 488 Neurotrophin 4 antibody (CL488-12297) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Neurotrophin 4 Polyclonal antibody

Neurotrophin 4 Polyclonal Antibody for IF/ICC
Cat No. CL488-12297

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

neurotrophin 4, Neurotrophin 5, Neutrophic factor 4, NT 4, NT 4/5, NT 5, NT4, NT-4, NT5, NTF4, NTF5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inPC-3 cells
For other applications, we recommend the unconjugated version of this antibody, 12297-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12297 targets Neurotrophin 4 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen Neurotrophin 4 fusion protein Ag2938 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name neurotrophin 4
Synonyms neurotrophin 4, Neurotrophin 5, Neutrophic factor 4, NT 4, NT 4/5, NT 5, NT4, NT-4, NT5, NTF4, NTF5
Calculated Molecular Weight 210 aa, 22 kDa
Observed Molecular Weight70-80 kDa
GenBank Accession NumberBC012421
Gene Symbol Neurotrophin 4
Gene ID (NCBI) 4909
RRIDAB_3672534
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP34130
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neurotrophin 4 (NT-4) belongs to the family of neurotrophic factors, and it interacts with the tyrosine kinase B (trkB) receptor. NT-4 has neuroprotective effects following cerebral ischemia. Its role might be similar to brain-derived neurotrophic factor (BDNF) because both interact with trkB. Exercise also improves neural function by increasing neurotrophic factors. NT-4 exists in two forms, either as a dimer (80 kDa) or as a monomer (40-47 kDa) (PMID: 23526925).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Neurotrophin 4 antibody CL488-12297Download protocol
Standard Protocols
Click here to view our Standard Protocols