NUCB2/nesfatin-1 Recombinant antibody
NUCB2/nesfatin-1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
241037C1
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | A431 cells, HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 83917-5-RR is NUCB2/nesfatin-1 Fusion Protein expressed in E. coli.
| Tested Applications | IF/ICC, FC (Intra), ELISA Application Description |
| Tested Reactivity | human |
| Immunogen | NUCB2/nesfatin-1 fusion protein Ag25152 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | nucleobindin 2 |
| Synonyms | NUCB2, nucleobindin 2, NEFA, Gastric cancer antigen Zg4, DNA binding protein NEFA |
| Calculated Molecular Weight | 50 kDa |
| GenBank Accession Number | NM_005013 |
| Gene Symbol | NUCB2 |
| Gene ID (NCBI) | 4925 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P80303 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NucB2, an anorexigenic molecule, is expressed mainly in the hypothalamus, particularly in the supraoptic nucleus (SON) and the paraventricular nucleus (PVN). NucB2 is also expressed in the subfornical organ (SFO). Because the SON and PVN are involved in body fluid regulation, NucB2 may be involved in dehydration-induced anorexia. NUCB2 is composed of a signal peptide of 24 amino acids in the N-terminal region and a protin structure containing 396 amino acids. Nesfatin-1 is an 82-amino-acid peptide hormone cleaved from nucleobindin2 (NUCB2).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for NUCB2/nesfatin-1 antibody 83917-5-RR | Download protocol |
| FC protocol for NUCB2/nesfatin-1 antibody 83917-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
