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验证数据展示

  • FC experiment of HepG2 using CL488-83917-5

    FC experiment of HepG2 using CL488-83917-5

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CoraLite® Plus 488-conjugated NUCB2/nesfatin-1 Recombinant antibody (CL488-83917-5, Clone:241037C1)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL488-83917-5

    IF Staining of HepG2 using CL488-83917-5

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 NUCB2/nesfatin-1 antibody (CL488-83917-5, Clone: 241037C1 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated NUCB2/nesfatin-1 Recombinant antibody

NUCB2/nesfatin-1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-83917-5

产品说明书

CloneNo. 241037C1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

NUCB2, 241037C1, DNA binding protein NEFA, Gastric cancer antigen Zg4, NEFA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells, HeLa cells
For other applications, we recommend the unconjugated version of this antibody, 83917-5-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83917-5 targets NUCB2/nesfatin-1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 NUCB2/nesfatin-1 fusion protein Ag25152 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nucleobindin 2
别名 NUCB2, 241037C1, DNA binding protein NEFA, Gastric cancer antigen Zg4, NEFA
计算分子量 50 kDa
GenBank蛋白编号NM_005013
基因名称 NUCB2
Gene ID (NCBI) 4925
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP80303
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NucB2, an anorexigenic molecule, is expressed mainly in the hypothalamus, particularly in the supraoptic nucleus (SON) and the paraventricular nucleus (PVN). NucB2 is also expressed in the subfornical organ (SFO). Because the SON and PVN are involved in body fluid regulation, NucB2 may be involved in dehydration-induced anorexia. NUCB2 is composed of a signal peptide of 24 amino acids in the N-terminal region and a protin structure containing 396 amino acids. Nesfatin-1 is an 82-amino-acid peptide hormone cleaved from nucleobindin2 (NUCB2).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NUCB2/nesfatin-1 antibody CL488-83917-5Download protocol
Standard Protocols
Click here to view our Standard Protocols