- Featured Product
- KD/KO Validated
NUMBL Polyclonal antibody
NUMBL Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, mouse spleen tissue, HeLa cells, mouse lung tissue, mouse liver tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human brain tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | PC-12 cells, HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 4 publications below |
IHC | See 3 publications below |
产品信息
10111-1-AP targets NUMBL in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | NUMBL fusion protein Ag0156 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | numb homolog (Drosophila)-like |
Synonyms | Numb-related protein, Numb-like protein, NUMBLIKE |
Calculated Molecular Weight | 65 kDa |
Observed Molecular Weight | 65-70 kDa |
GenBank Accession Number | BC001794 |
Gene Symbol | NUMBL |
Gene ID (NCBI) | 9253 |
RRID | AB_2154440 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9Y6R0 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for NUMBL antibody 10111-1-AP | Download protocol |
IHC protocol for NUMBL antibody 10111-1-AP | Download protocol |
IF protocol for NUMBL antibody 10111-1-AP | Download protocol |
IP protocol for NUMBL antibody 10111-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Neurosci Numb and Numbl are required for maintenance of cadherin-based adhesion and polarity of neural progenitors. | ||
Development Cleaved Delta like 1 intracellular domain regulates neural development via Notch signal-dependent and -independent pathways. | ||
Mol Endocrinol Numb deletion in POMC-expressing cells impairs pituitary intermediate lobe cell adhesion, progenitor cell localization, and neuro-intermediate lobe boundary formation. |