验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse spleen tissue, HeLa cells, mouse lung tissue, mouse liver tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human brain tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse cerebellum tissue |
| Positive IF/ICC detected in | PC-12 cells, HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy NUMBL Ready-To-Use IHC Kit. NUMBL primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IHC | See 3 publications below |
产品信息
10111-1-AP targets NUMBL in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | NUMBL fusion protein Ag0156 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | numb homolog (Drosophila)-like |
| 别名 | Numb-related protein, Numb-like protein, NUMBLIKE |
| 计算分子量 | 65 kDa |
| 观测分子量 | 65-70 kDa |
| GenBank蛋白编号 | BC001794 |
| 基因名称 | NUMBL |
| Gene ID (NCBI) | 9253 |
| RRID | AB_2154440 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y6R0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Numbl (also known as numblike or Nbl), as a conserved homolog of Drosophila Numb, is specifically expressed in the brain. Numbl is a cytoplasm protein and has redundant functions in embryonic neurogenesis. It plays an important role in ependymal wall integrity and subventricular zone neuroblast survival. However, its expression and function in the central nervous system lesion are still unclear.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NUMBL antibody 10111-1-AP | Download protocol |
| IHC protocol for NUMBL antibody 10111-1-AP | Download protocol |
| IF protocol for NUMBL antibody 10111-1-AP | Download protocol |
| IP protocol for NUMBL antibody 10111-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Neurosci Numb and Numbl are required for maintenance of cadherin-based adhesion and polarity of neural progenitors. | ||
Development Cleaved Delta like 1 intracellular domain regulates neural development via Notch signal-dependent and -independent pathways. | ||
Mol Endocrinol Numb deletion in POMC-expressing cells impairs pituitary intermediate lobe cell adhesion, progenitor cell localization, and neuro-intermediate lobe boundary formation. |
