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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated NUMBL Polyclonal antibody

NUMBL Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10111

Print datasheet

Synonyms

Numb-related protein, Numb-like protein, NUMBLIKE

验证数据展示

  • IF Staining of mouse cerebellum using CL488-10111

    IF Staining of mouse cerebellum using CL488-10111

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using CoraLite® Plus 488 NUMBL antibody (CL488-10111) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IF-P detected inmouse cerebellum tissue
Planning an IHC experiment? We recommend our IHCeasy NUMBL Ready-To-Use IHC Kit. NUMBL primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10111-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10111 targets NUMBL in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse, rat
Immunogen NUMBL fusion protein Ag0156 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name numb homolog (Drosophila)-like
Synonyms Numb-related protein, Numb-like protein, NUMBLIKE
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC001794
Gene Symbol NUMBL
Gene ID (NCBI) 9253
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6R0
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Numbl (also known as numblike or Nbl), as a conserved homolog of Drosophila Numb, is specifically expressed in the brain. Numbl is a cytoplasm protein and has redundant functions in embryonic neurogenesis. It plays an important role in ependymal wall integrity and subventricular zone neuroblast survival. However, its expression and function in the central nervous system lesion are still unclear.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NUMBL antibody CL488-10111Download protocol
Standard Protocols
Click here to view our Standard Protocols