- Featured Product
- KD/KO Validated
NUP155 Monoclonal antibody
NUP155 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, rat and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1A7F2
验证数据展示
经过测试的应用
| Positive WB detected in | HEK293 cells, HeLa cells, ROS1728 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
产品信息
66359-1-Ig targets NUP155 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | NUP155 fusion protein Ag3638 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | nucleoporin 155kDa |
| Synonyms | 155 kDa nucleoporin, KIAA0791, N155, nucleoporin 155kDa, Nucleoporin Nup155, NUP155 |
| Calculated Molecular Weight | 1391 aa, 155 kDa |
| Observed Molecular Weight | 140-155 kDa |
| GenBank Accession Number | BC039257 |
| Gene Symbol | NUP155 |
| Gene ID (NCBI) | 9631 |
| RRID | AB_2881738 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O75694 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NUP155 is a nucleoporin, the major component of the nuclear pore complex (NPC) that is essential for nuclear envelope biogenesis. Mutation of NUP155 has been linked to cardiovascular disease.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NUP155 antibody 66359-1-Ig | Download protocol |
| IHC protocol for NUP155 antibody 66359-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neuron Nucleoporin Seh1 Interacts with Olig2/Brd7 to Promote Oligodendrocyte Differentiation and Myelination. | ||
J Ethnopharmacol Xinfuli granule alleviates metabolic remodeling through inhibition of endoplasmic reticulum stress and mitochondrial injury in heart failure | ||
BMC Cancer Pan-cancer analysis of NUP155 and validation of its role in breast cancer cell proliferation, migration, and apoptosis
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