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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated NUP155 Monoclonal antibody

NUP155 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A7F2

Cat no : CL488-66359

Print datasheet

Synonyms

155 kDa nucleoporin, KIAA0791, N155, nucleoporin 155kDa, Nucleoporin Nup155, NUP155

验证数据展示

  • WB analysis using CL488-66359

    WB analysis using CL488-66359

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66359 (NUP155 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, K-562 cells
For other applications, we recommend the unconjugated version of this antibody, 66359-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66359 targets NUP155 in WB applications and shows reactivity with human, rat samples.

Tested Applications WB Application Description
Tested Reactivity human, rat
Immunogen NUP155 fusion protein Ag3638 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name nucleoporin 155kDa
Synonyms 155 kDa nucleoporin, KIAA0791, N155, nucleoporin 155kDa, Nucleoporin Nup155, NUP155
Calculated Molecular Weight 1391 aa, 155 kDa
Observed Molecular Weight 140-155 kDa
GenBank Accession NumberBC039257
Gene Symbol NUP155
Gene ID (NCBI) 9631
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75694
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NUP155 is a nucleoporin, the major component of the nuclear pore complex (NPC) that is essential for nuclear envelope biogenesis. Mutation of NUP155 has been linked to cardiovascular disease.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 NUP155 antibody CL488-66359Download protocol
Standard Protocols
Click here to view our Standard Protocols