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验证数据展示

  • WB analysis using 67001-1-Ig (same clone as 67001-1-PBS)

    WB analysis using 67001-1-Ig (same clone as 67001-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67001-1-Ig (NUP50 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67001-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67001-1-Ig (same clone as 67001-1-PBS)

    IHC staining of human breast cancer using 67001-1-Ig (same clone as 67001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67001-1-Ig (NUP50 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67001-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NUP50 antibody included
  • Cytometric bead array sample test of MP51516-1

    Cytometric bead array sample test of MP51516-1

    Cytometric bead array sample test of MP51516-1, NUP50 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67001-1-PBS. Detection antibody: 67001-2-PBS.

  • IHC staining of human breast cancer using 67001-1-Ig (same clone as 67001-1-PBS)

    IHC staining of human breast cancer using 67001-1-Ig (same clone as 67001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67001-1-Ig (NUP50 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67001-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NUP50 antibody included
  • WB analysis of HEK-293 using 67001-1-Ig (same clone as 67001-1-PBS)

    WB analysis of HEK-293 using 67001-1-Ig (same clone as 67001-1-PBS)

    WB result of NUP50 antibody (67001-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUP50 transfected HEK-293 cells. This data was developed using the same antibody clone with 67001-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51516-1

    Cytometric bead array standard curve of MP51516-1

    Cytometric bead array standard curve of MP51516-1, NUP50 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67001-1-PBS. Detection antibody: 67001-2-PBS. Standard:Ag15144. Range: 1.563-200 ng/mL.

  • Featured Product
  • KD/KO验证

NUP50 Monoclonal antibody, PBS Only (Capture)

NUP50 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 67001-1-PBS

产品说明书

CloneNo. 1D5A7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

1D5A7, 50 kDa nucleoporin, Nuclear pore-associated protein 60 kDa-like, nucleoporin 50kDa, Nucleoporin Nup50

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NUP50 Ready-To-Use IHC Kit. NUP50 primary antibody included.

产品信息

67001-1-PBS targets NUP50 as part of a matched antibody pair:

MP51516-1: 67001-1-PBS capture and 67001-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag15144

Product name: Recombinant human NUP50 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 141-440 aa of BC028125

Sequence: DWIVKHVNTNPLCDLTPIFKDYEKYLANIEQQHGNSGRNSESESNKVAAETQSPSLFGSTKLQQESTFLFHGNKTEDTPDKKMEVASEKKTDPSSLGATSASFNFGKKVDSSVLGSLSSVPLTGFSFSPGNSSLFGKDTTQSKPVSSPFPTKPLEGQAEGDSGECKGGDEEENDEPPKVVVTEVKEEDAFYSKKCKLFYKKDNEFKEKGIGTLHLKPTANQKTQLLVRADTNLGNILLNVLIPPNMPCTRTGKNNVLIVCVPNPPIDEKNATMPVTMLIRVKTSEDADELHKILLEKKDA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 nucleoporin 50kDa
别名 1D5A7, 50 kDa nucleoporin, Nuclear pore-associated protein 60 kDa-like, nucleoporin 50kDa, Nucleoporin Nup50
计算分子量 468 aa, 50 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC028125
基因名称 NUP50
Gene ID (NCBI) 10762
RRIDAB_2882318
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UKX7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nup50 (also known as Npap60) is a nucleoporin that binds directly to importin α and their interaction plays an important role in nuclear import. Nup50 is a mobile nucleoporin localized primarily in the nuclear basket of the nuclear pore complex (NPC) and in the nucleoplasm. Human Nup50 has two isoforms: Npap60L (1-469 aa), and its alternatively spliced isoform, Npap60S (29-469 aa) of Npap60L.

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