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  • Featured Product
  • KD/KO Validated

NUP50 Monoclonal antibody, PBS Only

NUP50 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D5A7

Cat no : 67001-1-PBS

Print datasheet

Synonyms

50 kDa nucleoporin, nucleoporin 50kDa, Nucleoporin Nup50, NUP50

验证数据展示

  • WB analysis using 67001-1-Ig (same clone as 67001-1-PBS)

    WB analysis using 67001-1-Ig (same clone as 67001-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67001-1-Ig (NUP50 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67001-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67001-1-Ig (same clone as 67001-1-PBS)

    IHC staining of human breast cancer using 67001-1-Ig (same clone as 67001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67001-1-Ig (NUP50 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67001-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NUP50 antibody included
  • IHC staining of human breast cancer using 67001-1-Ig (same clone as 67001-1-PBS)

    IHC staining of human breast cancer using 67001-1-Ig (same clone as 67001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67001-1-Ig (NUP50 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67001-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NUP50 antibody included
  • WB analysis of HEK-293 using 67001-1-Ig (same clone as 67001-1-PBS)

    WB analysis of HEK-293 using 67001-1-Ig (same clone as 67001-1-PBS)

    WB result of NUP50 antibody (67001-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUP50 transfected HEK-293 cells. This data was developed using the same antibody clone with 67001-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy NUP50 Ready-To-Use IHC Kit. NUP50 primary antibody included.

产品信息

67001-1-PBS targets NUP50 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, mouse
Immunogen NUP50 fusion protein Ag15144 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nucleoporin 50kDa
Synonyms 50 kDa nucleoporin, nucleoporin 50kDa, Nucleoporin Nup50, NUP50
Calculated Molecular Weight 468 aa, 50 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC028125
Gene Symbol NUP50
Gene ID (NCBI) 10762
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UKX7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nup50 (also known as Npap60) is a nucleoporin that binds directly to importin α and their interaction plays an important role in nuclear import. Nup50 is a mobile nucleoporin localized primarily in the nuclear basket of the nuclear pore complex (NPC) and in the nucleoplasm. Human Nup50 has two isoforms: Npap60L (1-469 aa), and its alternatively spliced isoform, Npap60S (29-469 aa) of Npap60L.