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NUP50 Monoclonal antibody

NUP50 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D5A7

Cat no : 67001-1-Ig

Print datasheet

Synonyms

50 kDa nucleoporin, nucleoporin 50kDa, Nucleoporin Nup50, NUP50

验证数据展示

  • WB analysis using 67001-1-Ig

    WB analysis using 67001-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67001-1-Ig (NUP50 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human breast cancer using 67001-1-Ig

    IHC staining of human breast cancer using 67001-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67001-1-Ig (NUP50 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUP50 antibody included
  • IHC staining of human breast cancer using 67001-1-Ig

    IHC staining of human breast cancer using 67001-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67001-1-Ig (NUP50 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUP50 antibody included
  • WB analysis of HEK-293 using 67001-1-Ig

    WB analysis of HEK-293 using 67001-1-Ig

    WB result of NUP50 antibody (67001-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUP50 transfected HEK-293 cells.

经过测试的应用

Positive WB detected inHCT 116 cells, HeLa cells, HEK-293 cells, Ramos cells, Daudi cels, Sp2/0 cells, MCF-7 cells, HT-29 cells, U2OS cells, HepG2 cells, Jurkat cells, MOLT-4 cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NUP50 Ready-To-Use IHC Kit. NUP50 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67001-1-Ig targets NUP50 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen NUP50 fusion protein Ag15144 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nucleoporin 50kDa
Synonyms 50 kDa nucleoporin, nucleoporin 50kDa, Nucleoporin Nup50, NUP50
Calculated Molecular Weight 468 aa, 50 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC028125
Gene Symbol NUP50
Gene ID (NCBI) 10762
RRIDAB_2882318
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UKX7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nup50 (also known as Npap60) is a nucleoporin that binds directly to importin α and their interaction plays an important role in nuclear import. Nup50 is a mobile nucleoporin localized primarily in the nuclear basket of the nuclear pore complex (NPC) and in the nucleoplasm. Human Nup50 has two isoforms: Npap60L (1-469 aa), and its alternatively spliced isoform, Npap60S (29-469 aa) of Npap60L.

实验方案

Product Specific Protocols
WB protocol for NUP50 antibody 67001-1-IgDownload protocol
IHC protocol for NUP50 antibody 67001-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols