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验证数据展示

  • FC experiment of HL-60 using CL488-67001

    FC experiment of HL-60 using CL488-67001

    1X10^6 HL-60 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NUP50 (CL488-67001, Clone:1D5A7) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated NUP50 Monoclonal antibody

NUP50 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67001

产品说明书

CloneNo. 1D5A7

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

FC (Intra)

别名

PRO1146, Nucleoporin Nup50, nucleoporin 50kDa, Nuclear pore-associated protein 60 kDa-like, 50 kDa nucleoporin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHL-60 cells
Planning an IHC experiment? We recommend our IHCeasy NUP50 Ready-To-Use IHC Kit. NUP50 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67001-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67001 targets NUP50 in FC (Intra) applications and shows reactivity with Human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human
免疫原 NUP50 fusion protein Ag15144 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 nucleoporin 50kDa
别名 PRO1146, Nucleoporin Nup50, nucleoporin 50kDa, Nuclear pore-associated protein 60 kDa-like, 50 kDa nucleoporin
计算分子量 468 aa, 50 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC028125
基因名称 NUP50
Gene ID (NCBI) 10762
RRIDAB_3084296
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9UKX7
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nup50 (also known as Npap60) is a nucleoporin that binds directly to importin α and their interaction plays an important role in nuclear import. Nup50 is a mobile nucleoporin localized primarily in the nuclear basket of the nuclear pore complex (NPC) and in the nucleoplasm. Human Nup50 has two isoforms: Npap60L (1-469 aa), and its alternatively spliced isoform, Npap60S (29-469 aa) of Npap60L.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 NUP50 antibody CL488-67001Download protocol
Standard Protocols
Click here to view our Standard Protocols