验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, human liver tissue, L02 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
16587-1-AP targets NUPL2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | NUPL2 fusion protein Ag8816 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nucleoporin like 2 |
| 别名 | CG1, hCG1, NLP 1, NLP_1, NLP1, Nucleoporin hCG1, nucleoporin like 2, Nucleoporin like protein 2, NUP42 homolog, NUPL2 |
| 计算分子量 | 45 kDa |
| 观测分子量 | 45 kDa, 33-36 kDa |
| GenBank蛋白编号 | BC005327 |
| 基因名称 | NUPL2 |
| Gene ID (NCBI) | 11097 |
| RRID | AB_2267702 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15504 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NUPL2 (also known as NLP-1, or hCG1) is an ubiquitous nuclear protein containing many phenylalanine-glycine (FG) repeats. As the functional orthologue of the budding yeast Nup42/Rip1 nucleoporin, NUPL2 is required for the export of mRNAs containing poly(A) tails from the nucleus into the cytoplasm (PMID: 10610322). In case of infection by HIV-1, it may participate in the docking of viral Vpr at the nuclear envelope (PMID: 16000379). NUPL2 localizes to the nuclear envelope and could also be mobile within the nucleus. It promotes CRM1-dependent nuclear protein export (PMID: 22250199).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NUPL2 antibody 16587-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
