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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated NUSAP1 Polyclonal antibody

NUSAP1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12024

Print datasheet

Synonyms

ANKT, BM037, FLJ13421, LNP, NuSAP, NUSAP1, PRO0310, PRO0310p1, Q0310, SAPL

验证数据展示

  • IF Staining of HeLa using CL488-12024

    IF Staining of HeLa using CL488-12024

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 NUSAP1 antibody (CL488-12024) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 12024-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12024 targets NUSAP1 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen NUSAP1 fusion protein Ag2654 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nucleolar and spindle associated protein 1
Synonyms ANKT, BM037, FLJ13421, LNP, NuSAP, NUSAP1, PRO0310, PRO0310p1, Q0310, SAPL
Calculated Molecular Weight 440 aa, 49 kDa
Observed Molecular Weight 47-52 kDa
GenBank Accession NumberBC001308
Gene Symbol NUSAP1
Gene ID (NCBI) 51203
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BXS6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NUSAP1, also named as ANKT, belongs to the NUSAP family. It is microtubule-associated protein with the capacity to bundle and stabilize microtubules. NUSAP1 may associate with chromosomes and promote the organization of mitotic spindle microtubules around them. NUSAP1 plays a role in connecting apoptosis with cell migration and it is a potentially involved in morphogenesis in vertebrates (PMID:21203972). NUSAP1 is an immunogenic antigen in 65% of patients with AML following allogeneic HCT and suggests a tumor antigen role(PMID:20053754 ). NuSAP1 protein level is tightly regulated by the APC/C ubiquitin ligase complex and NuSAP induces mitotic arrest dependent of its microtubule affinity (PMID:17618083). NUSAP1 is overexpressed in pituitary adenomas.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NUSAP1 antibody CL488-12024Download protocol
Standard Protocols
Click here to view our Standard Protocols